Bovine type I procollagen N-terminal propeptide (PINP) ELISA kit

Bovine type I procollagen N-terminal propeptide (PINP) ELISA kit

Price: 2600

Double antibody sandwich ELISA method

[Specification] 96T (detecting 84 samples)/48T (detecting 36 samples)

Humans, rats, mice, pigs, chickens, plants, rabbits, monkeys, etc

[ELISA Kit Product Usage]

For scientific research purposes only, to detect the activity or concentration of relevant indicators in serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other related samples.

Storage conditions and expiration date

1. Store at 2-8 ℃, do not freeze, expiration date is 6 months.

2. After opening and using, the package is wrapped in a microporous plate and placed in a self sealing bag with a desiccant. The self sealing bag is sealed and all reagents are placed back in the 2-8 ℃ refrigerator.

operating steps

1. Sample addition: Set up standard wells and blank wells respectively (blank control wells do not add samples or enzyme-linked immunosorbent assay reagents, the rest of the steps are the same)Sample hole to be testedAdd to the standard well on the enzyme-linked immunosorbent assay (ELISA) coated plate50Microliters, add sample diluent to the well of the sample to be tested first40MlThen add the sample to be tested10MlThe final dilution of the sample is5Double).Add the sample to the bottom of the enzyme-linked immunosorbent assay plate well, avoiding contact with the well wall as much as possible,gentlyshakemix well.

2. Enzyme addition: Add enzyme labeled reagent to each well50MlExcept for blank holes.

3. Incubation: Seal the plate with a sealing film and place it on the back37Incubate at 6 ℃0minute.

4. Liquid preparation: will30Concentrated washing solution with distilled water30Dilute twice before use

5. Washing: Carefully remove the sealing film and discard the liquid,spin-dryFill each hole with detergent and let it stand30Abandon in seconds, repeat like this5Next time, shoot dry.

6. Color development: Add color developer to each hole firstA50MlAdd color developer againB50MlGently shake and mix well,37Color development in the dark at ℃ for 15 minutes

7. Termination: Add termination for each holeliquid50μlTerminate the reaction(At this moment, blue turns to yellow).

8. Measurement: Zero with blank hole,450nmMeasure the wavelengths of each hole in sequenceabsorb lightDegree（ODValue). The measurement should be conducted after adding the termination solution15Conducted within minutes.