Pine wood nematode rapid detection instrument

1、 Instrument usage

　　Pine wood nematode rapid detection instrumentThe main processes include isolation of pine wood nematodes, DNA extraction, and fluorescence quantitative PCR detection. It can quickly complete the detection of pine wood nematodes, automatically display the detection results and print the detection report, without the need for manual interpretation, with an accuracy rate of over 99%, greatly improving the efficiency of pine wood nematode detection work. The field rapid detection system is equipped with a portable and mobile operation, providing convenience for the detection of pine wood nematodes in the field. The automated molecular detection system for pine wood nematodes is widely used in forest defense stations, customs, universities, research institutions, and other units.

2、 Instrument features

1. The system has high detection accuracy.

2. The intelligent pine wood nematode DNA rapid detection system is equipped with an automated detection program for pine wood nematode DNA, which does not require complicated program settings and can run automatically with one click. It can not only detect nematode separation solution, but also directly detect pine wood chips without separating nematodes.

3. The system detection task is easy to operate, time-saving and efficient, and suitable for forestry and forest defense departments at all levels. It only takes 60 minutes to complete the entire process including sample processing, DNA extraction, computer-based testing, and result interpretation.

4. By using amplification fluorescence technology, laboratory testing and identification requirements can be completed through the system host.

5. Small size, light weight, and easy to carry. Easily meet the needs of outdoor experiments.

6. Built in 10 inch high-definition capacitive screen PDA, touch screen operation, simple and fast.

7. Marlow high-quality Peltier refrigeration chips, combined with German PT1000 temperature sensor and temperature control mode with electric resistance heating compensation edge, have a heating rate of 7 ℃ and a cooling rate of 5 ℃, greatly reducing the experimental time.

8. The whole board adopts a 3-second fast lighting mode to ensure the consistency of the hole positions in the experimental results.

9. Simple and intuitive software guidance, making it easy to start detection experiments.

3、 Technical parameters

1. High specification aluminum alloy sample slots are used, with good thermal conductivity, corrosion and oxidation resistance, Peltier heating and temperature control, and comprehensive sealing design. Both the sample solution and condensed water will not leak into the Peltier temperature control element and other electronic components below the sample tank, effectively extending the service life of the instrument.

2. Adopting high-precision PT1000 temperature sensor, the temperature control accuracy reaches 0.01 ℃, fully ensuring the accuracy of temperature control.

3. Sample flux: 0.2ml single tube, 0.2ml eight tube.

4. Open reagent consumables system: Any brand of reagent can be selected, suitable for standard 8-tube or 0.2ml PCR single tube

5. Four high-intensity, wide spectrum, long-life LED solid-state light sources, blue, green, red, and yellow, are used to ensure strong light intensity throughout the entire spectral detection range, improve detection sensitivity, and do not generate heat. They have a long service life and are maintenance free for life.

6. High sensitivity detector: Using a highly sensitive photomultiplier tube imported from Japan, the detection sensitivity is even higher.

7. No need for optical path correction: The scanning system can ensure that the signal excitation and detection optical path of each hole are consistent, without the need for optical path correction, so there is no need to occupy an additional correction channel, and true four channel gene detection can be achieved. ★

8. Number of channels: Configure 4 channels.

9. Channel crosstalk: High precision narrow band filters are used to greatly reduce channel crosstalk, and software can also correct crosstalk parameters.

10. Detection sensitivity: It can detect single copy DNA templates and clearly distinguish between 1000 copy and 2000 copy plasmid DNA

11. Linear detection range: 10 orders of magnitude ★

12. Support commonly used fluorescent dyes and probe detection methods, such as SYBR Green I，Eva Green，Taqman，FRET，Molecular Beacons，Scorpions，Amplifluor Wait

13. Module temperature range: 4-99 ℃

14. Control mode: PCR operating system built-in to the system screen or Windows computer ★

15. Interface: Computer USB connection

16. Working noise: The working noise during operation does not exceed 60 dB

17. Operation analysis software

17.1 It is possible to define sample holes after running the program, saving experimental time

17.2 When running the program, other experimental data results can be opened simultaneously for review and analysis

17.3 The software automatically calculates the baseline range and sets the threshold line, providing users with reliable and accurate data results

17.4 Experimental program setting information and detection results can be directly printed as experimental reports, supporting multiple file formats and data styles such as XLS, CSV, WORD for export ★

17.5 The results of the same batch of experiments can be grouped for independent data analysis, up to a maximum of 6 groups

17.6 Provide software interfaces in both Chinese and English. ★

17.7 software can be installed on multiple computers without any restrictions, without the need to purchase usage rights, and can be upgraded for free for life. ★

　　Pine wood nematode rapid detection instrumentInstrument technical specifications