Photochemical derivatizer

Photochemical derivatizerPrinciples and advantages

Aspergillus flavus B1 and G1 themselves have strong fluorescence, but in aqueous solvents, fluorescence quenching occurs, greatly reducing their fluorescence. After derivation, with the corresponding increase in fluorescence intensity, the sensitivity of the instrument also greatly improves.

Compared to the same column front derivation method:Photochemical derivatizerIt has the advantages of easy operation, strong uniformity, and accurate results.

Compared with electrochemical derivatizers, it has advantages such as no need for additional reagents, better instrument maintenance, stronger uniformity, lower instrument cost, and less environmental pollution.

Compliant with the 2015 edition pharmacopoeia and tested for Aspergillus flavus in traditional Chinese medicine.

The timed shutdown function is more suitable for use with highly automated systems.

Scope of application

Photochemical derivatizerWidely used in HPLC detection of sulfonamide drugs, it can enhance the fluorescence intensity of Aspergillus flavus B1 and G1, and the sensitivity of Aspergillus flavus B1 and G1 can reach 0.1 ppb or higher; It can also enhance the fluorescence intensity of sulfonamide drugs and improve the sensitivity of HPLC detection.

Photochemical derivatizerFeatures:

1. This onePhotochemical derivatizerNo need for any chemical reagents, no consumption of derivatization reagents, low cost;

2. No need for chemical derivative pumps, mixers, temperature control devices, low equipment investment costs;

3. Directly connected between the chromatographic column and the fluorescence detector, with a small footprint and simple operation;

4. Derivatives can enhance the fluorescence, reactivity, and sensitivity of Aspergillus flavus B1 and G1;

5. Non corrosive acid flows through the capillary, increasing the lifespan of the HPLC instrument;

6. Can work continuously for thousands of hours without the need for rinsing steps after use;

7. The flow rate can reach 2ml/minute, and other flow rates can be customized;

8. The derivation time is greatly reduced, and ultra efficient UPLC can be completed in 5 minutes;

9. The low detection limits of Aspergillus flavus B1, B2, G1, and G2 are all less than 0.5 ppb;

10. It can also be applied for HPLC detection of sulfonamide drugs, with a sensitivity of up to 10 ppb;

11. Also applicable for analysis of barbiturates, amino acids, peptides, and vitamins;

12. Photoderivatization results and electrochemical methods (iodine derivatization) *;

13. Meets the requirements of AOAC 2005.08, AOAC 2008.02, and AOCS Aa 11-05;

14. Meet the requirements of Taiwan, China Standard (SZ 0981800370);

15. Complies with the analytical method requirements of EU Pharmacopoeia 2.8.18 standard;

16. Complies with the requirements of the 2015 edition of the Chinese Pharmacopoeia (with the addition of photochemical derivatization method);

17. It is compatible with the HPLC fluorescence detector of Shanghai Kezhe Biochemical Company;

18. Can be paired with the same casing as the HPLC fluorescence detector from Shanghai Kezhe Company to maintain its appearance;

19. Light intensity adjustment devices can be added to adjust different light intensities;

20. Automated time control devices can be added for equipment switch management;

21. Light source lifespan management software can be added for instrument usage management;

【 Precautions 】

Working power supply: 220V, 50Hz

Working power: 11W

UV lamp: specially designed U-shaped tube

Pipeline arrangement method: Three dimensional orderly and neat arrangement, ensuring more sufficient UV irradiation and more efficient derivation;

Rated pressure: 2500psi ≈ 170bar ≈ 17MPa

Environmental temperature: 0~45 ℃

Environmental requirements: It is strictly prohibited to immerse or water, and fragile substances should be contained to avoid impact and falling.

Non maintenance personnel are strictly prohibited from disassembling the instrument. There is ultraviolet light inside, and do not look directly at the leaking parts.

The lamp tube is a lamp tube, please make sure to cut off the power when replacing it.

The instrument naturally dissipates heat. Please ensure ventilation during operation and do not cover it to affect heat dissipation.