PCR nucleic acid testing laboratory hospital purification engineering design and construction

PCR nucleic acid testing laboratory hospital purification engineering design and construction

key pointsPCR testing laboratoryIt can be in a dispersed form or a combined form. Completing a set of PCR detection experiments usually involves four experimental processes: reagent preparation, sample processing, nucleic acid amplification, and product analysis. If the experimental process requires it, a sample crushing process should also be added.1、 Dispersed form PCR detection laboratoryThe so-called dispersed form PCR detection laboratory refers to the experimental rooms that are far apart from each other and arranged in a dispersed manner to complete the above experimental process. For this arrangement of PCR detection laboratory, there is no need for special conditions as the experiments are not easily interfered with each other.2、 Combination form PCR detection laboratoryThe so-called combination form PCR detection laboratory refers to the form in which the experimental rooms for completing the four experimental processes of PCR are arranged adjacent to each other, forming an independent experimental area. For the combinatorial PCR laboratory, due to the centralized arrangement of various experiments, it is easy to cause mutual interference, therefore, there are certain requirements for the overall layout and barrier system. Each room is equipped with a buffer room at the entrance to reduce indoor and outdoor air exchange.

PCR nucleic acid testing laboratory hospital purification engineering design and construction

Some experiences:The reagent preparation room and sample processing room should have a slight positive pressure to prevent air containing nucleic acid aerosols from entering and causing pollution;Positive pressure can be achieved by controlling the intake air volume to be greater than the exhaust air volume.The nucleic acid amplification room and product analysis room should have a slight negative pressure to prevent aerosols containing nucleic acids from diffusing and contaminating reagents and samples. The negative pressure effect can be achieved by controlling the exhaust air volume to be greater than the inlet air volume.In an ideal situation, a positive pressure can be set in the buffer room of the PCR detection laboratory to prevent indoor air from flowing outside and outdoor air from flowing indoors. The air intake of the PCR laboratory is controlled by the original central air conditioning system, and it is required to install the central air conditioning vents in placeAnd the height is 2600mm after the ground is paved.If a fluorescent PCR instrument is used, the amplification chamber and product analysis chamber can be merged.If the room depth allows, a dedicated PCR internal corridor can be set up. It should be pointed out that buffer rooms are more meaningful than dedicated corridors in reducing indoor and outdoor air exchange.There should be unidirectional experimental process flow, logistics, pedestrian flow, and air flow between different regions to form a protective barrier for the unidirectional process, avoid mutual interference between experiments, and prevent nucleic acid aerosols from contaminating the experimental process and producing false results.The walls of the laboratory, including the ceiling, should have a sturdy structure and good air tightness; All corners should be transitioned with circular arc lines; The inner wall of the wall is smooth, non absorbent, corrosion-resistant, and easy to clean and disinfect; The ground material should meet the requirements of seamless, leak free, smooth, and corrosion-resistant.

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