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Yuxiu Biotechnology (Shanghai) Co., Ltd
Organize chip services
NegotiableUpdate on 05/06
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Overview
Tissue microarray services, also known as tissue microarrays, are an important branch of biochar technology that involves arranging various individual tissue specimens in a regular array on the same glass slide for in situ histological studies using the same index. Since its introduction in 1998, this technology has been widely promoted and applied due to its advantages of large-scale, high-throughput, and standardization. The reason is that the experimental conditions of the tissue samples on the chip are consistent, and some are subject to quality control.
Product Details
Organize chip services(tissuechip)， Also known as tissue microarrays, it is an important branch of biochar technology that arranges many different individual tissue specimens in a regular array on the same glass slide for in situ histological study of the same index in a Chemicalbook. Since its introduction in 1998, this technology has been widely promoted and applied due to its advantages of large-scale, high-throughput, and standardization. The reason is that the experimental conditions of the tissue samples on the chip are consistent, and some are subject to quality control.
Tissue Microarray (TMA) is a high-throughput biotechnology tool that achieves "one experiment, batch analysis" by accurately arranging hundreds or thousands of tiny tissue samples (diameter 0.6-2mm) onto the same paraffin or frozen carrier.
Traditional pathological section: 1 slice=1 sample → time-consuming, costly, and with large batch differences.
Organizational chip: 1 slice=hundreds of samples → Efficiency improved by hundreds of times, data comparability stronger.
Organize chip servicescore principle
1. Technical process
Sample collection: Drill cylindrical small samples (cores) from donor tissues (such as tumors, normal tissues).
Array construction: Embed samples into blank paraffin blocks according to preset coordinates to form an ordered array (similar to a "honeycomb coal" structure).
Slice preparation: Cut TMA blocks into 4-5 μ m thin slices for staining, immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and other detection methods.
2. Key technical points
Accurate positioning: Ensure precise sample position (error<50 μ m) through automated punching machine.
Quality control: HE staining is used to verify the integrity of the sample and exclude areas of necrosis or detachment.
Data association: Each sample corresponds to an independent ID and is linked to clinical information databases (such as survival period and pathological grading) for analysis.
Core application scenarios of organizational chips
1. Biomarker screening and validation
Cancer research: Batch detection of specific proteins (such as PD-L1, HER2) expression in tumor tissues, associated with patient prognosis.
Case: TMA was used to verify the positive correlation between Ki-67 overexpression and sensitivity in breast cancer.
Drug development: Screening the distribution of candidate drug targets in hundreds of samples to guide indication selection.
2. Standardization of molecular pathological diagnosis
Laboratory quality control: The same TMA slide can simultaneously detect staining consistency in multiple laboratories (such as EGFR mutation detection).
Development of diagnostic kits: Evaluate the specificity and sensitivity of antibodies/probes (such as ALK fusion gene FISH probes).
3. Big data and AI model training
Digital pathology: Scan TMA slices to generate panoramic images for AI algorithms to learn tissue morphology features.
Case: Training an AI model to predict microsatellite instability (MSI) in colorectal cancer using TMA data. In this way, the same set of tissue chips can quickly analyze and detect hundreds of biological molecular markers (such as antigens, DNA, and RNA) Therefore, tissue chip technology is a powerful tool for establishing biological molecular libraries of diseases, especially tumors.
Advantages:
1. Small size, high information content, and a large number of results can be obtained in one experiment
2. Can be used for localization analysis and detection of DNA, RNA, and proteins in tissues
3. Similar to regular tissue sections, HE staining, immunohistochemistry staining, DNA and RNA in situ hybridization can be performed.
4. Wax blocks can be sliced into 100-200 consecutive sections to improve sample utilization
In addition to the application scope described above, the application scenarios of organizational chips also include:
① Cell phenotype analysis: By using tissue chips and immunohistochemistry techniques, the expression of genes in various tissue lesions is detected, and cell phenotypes are analyzed;
② Disease gene screening: Combined with gene chips, after screening candidate genes using gene chips, fluorescence in situ hybridization verification is performed using tissue chips to discover genes related to diseases [1].
③ Research on Tumor Biomarkers: Through tissue chip detection of lesion samples at different stages, molecular markers related to tumor occurrence, development, and prognosis are discovered to assist in tumor molecular typing and personalized treatment.
④ Chemical composition detection: Quickly detect the chemical composition of tissues and cells, suitable for large sample analysis.
⑤ Biological reagent testing: Efficient screening and validation of antibodies and probes for specificity and sensitivity, superior to traditional pathological methods.
Process Introduction
By using the method of fine needle punching in tissue chip making machines, dozens to hundreds of cylindrical small tissues (tissue cores) are collected from numerous tissue wax blocks (known as donor wax blocks), and neatly arranged in another blank wax block (known as recipient wax block) to make tissue chip wax blocks. Then slice the wax block of the tissue chip, and transfer the slice to a glass slide to make the tissue chip.
The specific process is as follows:
① Chip micro display design
② Collect case slices and related wax blocks
③ Read the video and mark it accordingly
④ Preparation of TMA receptor wax block and determination of pore size
⑤ Transfer the tissue core into the well of the receptor module
⑥ Fixed wax melting
⑦ Slice staining
