Nitric oxide synthase typing activity detection kit

Nitric oxide synthase typing activity detection kit $r $n Nitric oxide synthase can catalyze L-arginine, molecular oxygen, and NADPH to generate NO and NADP+. NO is easily oxidized in aqueous solution to generate NO2- and NO3-; Under acidic conditions, NO2- reacts with diazonium sulfonamide to form diazonium compounds, which further couple with naphthyl vinyl diamine. The product exhibits a characteristic absorption peak at 550 nm, and the activity of nitric oxide synthase can be characterized by changes in absorbance values.

Boxbio®Nitric Oxide Synthase (NOS) Activity Typed Assay Kit

Classification of nitric oxide synthase（TNOSTheiNOSTheCNOS）Activity detection kit

Nitric oxide synthaseIt is an intracellular catalysis of L-jingAn enzyme that synthesizes nitric oxide from amino acids, widely present in vascular smooth muscle, macrophages, and other cells. As a cellular information molecule, it plays an important regulatory role in the nervous system, immune system, and cardiovascular system.

According to their different dependence on calcium ions for enzyme activity, NOS can be classified into structural types（ cNOS）And damage inducible NOS（iNOS）. Nitric oxide synthase can catalyze L-jingAmino acid, molecular oxygen, and NADPHGenerate NOAnd NADP+，NOHighly prone to oxidation and formation of NO in aqueous solution2-And NO3-Under acidic conditions, NO2-Generate diazo compounds with diazonium salt sulfonic acid amine, further couple with naphthyl vinyl diamine, and the product is at 550 nmThere is a characteristic absorption peak at this point, and the activity of nitric oxide synthase can be characterized by changes in absorbance values.

Nitric oxide synthase typing activity detection kitMeasurement processRequired instruments and reagents for:Visible spectrophotometer/Enzyme linked immunosorbent assay (ELISA) reader, micro glass colorimetric dish (optical path 10 mm)）/96Orifice plate, mortar/Homogenizer, adjustable pipette, desktop centrifuge, constant temperature water bath/Incubator and distilled water.

Preparation of crude enzyme solution (sample size and proportion can be adjusted appropriately according to the preliminary experimental results)

①Organization: Weigh 0.2 gOrganize, add 980 μ LExtract AAnd 20 μ LExtract B, ice bath homogenate, 12000 g at 4 ℃Centrifuge for 10 minutesTake the supernatant to obtainCrude enzyme solutionPlace it on ice for testing.

②Bacteria or cells: Collect 1000 by centrifugationTransfer 10000 bacteria or cells into a centrifuge tube and add 980 μ LExtract AAnd 20 μ LExtract BIce bath ultrasonic fragmentation (power 200 W)Ultrasound for 3 seconds, with an interval of 7 secondsThe total time is 5 minutes），4℃ 12000 gCentrifuge for 10 minutesTake the supernatant to obtainCrude enzyme solutionPlace it on ice for testing.

③Liquid samples such as culture medium: direct detection or appropriate dilution before detection. If turbid, appropriate centrifugation should be used to collect the supernatant for measurement.

Nitric oxide synthase typing activity detection kitPrecautions during the measurement process

① Nitric oxide synthase is prone to denaturation and inactivation. It is recommended to use fresh samples for detection, and the detection should be completed within days after the preparation of crude enzyme solution;

②After the preparation of reagent 2, it is recommended to take the required reagent 2 according to the usage amount, and the remaining reagent should be placed at -20 ℃ as soon as possiblepreservation;

③If ∆ AMeasurement greater than 0.4It is recommended to dilute the crude enzyme solution appropriately before conducting the measurement; If ∆ AMeasure less than 0.02It is recommended to extend the enzymatic reaction time appropriately (first step at 37 ℃)Measure after increasing the reaction time or sample size, and modify the calculation accordingly;

④To ensure accurate results and avoid reagent loss, please carefully read the instructions before testing (based on the actual received instructions), confirm whether the reagent storage and preparation are sufficient, whether the operating steps are clear, and be sure to take 2-3For samples with significant expected differences, please contact the staff promptly if there are any issues during the pre testing process.

Pre purchase notice:

A. Determine the type, specifications, and quantity of reagent kits based on the indicators to be tested;

B. Confirm whether the testing equipment and conditions match;

C. Confirm whether the delivery time and receipt time meet the requirements;

★Please contact Boxbio customer service or sales specialist promptly for any questions before purchasing

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D. For product quality issues, please provide a written explanation and detailed test results for reference within 3 months from the date of receipt of the product. If you do not raise any objections to the product quality within the above period, it will be deemed that you acknowledge the product as qualified;

E. The expiration date of the BOXBIO reagent kit is 3-6 months. If the product is used unopened after the expiration date, we can exchange the product for you free of charge;

F. The express delivery costs incurred for returns and exchanges due to product quality issues will be borne by our company.

Product Customization

Boxbio provides customized services for large packaging products:

Regarding the price:The salesperson will communicate with you, inquire about the customized reagent kit you need, and negotiate the required specifications based on the sample size to be tested, and then provide you with a quotation.

Regarding packaging:Production and warehouse delivery will match the corresponding packaging according to the specifications of the reagent kit you have customized. As it is a customized product, the packaging provided may not be of regular specifications. Please be aware.

Regarding delivery time:The delivery time for customized products will be calculated based on the type and specifications of the reagent kit you have customized, and will be communicated to you by sales personnel.

Research Assistance Fund

Boxbio is committed to making scientific research easier. In order to encourage and support academic research by researchers, we have launched the "Research Assistance Fund" reward program! Any SCI article published that indicates that the product is provided by Beijing Boxbio Science&Technology Co., Ltd. can apply for the corresponding research assistance fund!

According to the journal impact factor, the reward rules are as follows:

⑴Journal impact factor: 0<IF<5, research assistance fund amount is 300 yuan;

⑵Journal impact factor: 5 ≤ IF ＜ 10, research assistance fund amount is 800 yuan;

⑶Journal impact factor: 10 ≤ IF ＜ 15, research assistance fund amount is 1500 yuan;

⑷Journal impact factor: 15 ≤ IF ＜ 20, research assistance fund amount is 2500 yuan;

⑸Journal impact factor: 20 ≤ IF, research assistance fund amount is 3500 yuan.

*Non SCI articles can apply for a beautiful gift and receive corresponding points.

Application Instructions:

⑴The article must clearly indicate that the corresponding product is sourced from Beijing Box Biotechnology Co., Ltd. or Beijing Boxbio Science ＆ Technology Co.,Ltd.；

⑵The reward is only available to the first author of the article, and co authors can only apply for one reward;

⑶The same article can only apply for research funding once;

⑷If the products cited in the literature are trial equipment, they will not participate in this activity;

⑸The interpretation rights of this event belong to Beijing Box Biotechnology Co., Ltd.

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