Creatine content detection kit (Acetyl - α - Naphthol method)

Boxbio®Creatine Content Assay Kit (Diacetyl-α-Naphthol Method)

Creatine content detection kit (diacetyl)-a-Naphthol method)

creatineIt is a nitrogen-containing compound that naturally exists in the bodies of vertebrates, mainly composed of three amino groups: arginine suan, glycine suan, and methionine suanIt is composed of acids and can combine with phosphoric acid to form phosphocreatine, which is stored in muscle cells to provide energy for muscles and nerve cells. The changes in its content are closely related to muscle mass and function, and have important applications in energy metabolism, disease analysis, and nutritional research.

Creatine can react with acetyl - α under alkaline conditions-Naphthol reacts to produce a red compound, with the product at 530 nmThere is a characteristic absorption peak at this point, and the content of creatine can be quantitatively detected by changing the absorbance value.

Creatine content detection kit (Acetyl - α - Naphthol method)Instruments and reagents required during the measurement process:Visible spectrophotometer, 1 mLGlass colorimetric dish (optical diameter 10mm)）, mortar/Homogenizer, adjustable pipette, desktop centrifuge, and distilled water.

Sample processing (sample size and proportion can be adjusted appropriately based on pre experimental results)

①Organization: According to organizational quality (g)）Extract AVolume (mL)）For 1:（5-10）The ratio (recommended to weigh 0.1 g)Organize, add 1 mLExtract A）Sample processing, ice bath homogenate, 12000 g at 4 ℃Centrifuge for 10 minutes， 吸取 800 μLTransfer the supernatant to a centrifuge tube and add 150 μ LExtract BMix thoroughly, 12000 g at 4 ℃Centrifuge for 10 minutesTake the supernatant and place it on ice for testing.

②Bacteria or cells: Collect bacteria or cells by centrifugation into a centrifuge tube, according to the number of bacteria or cells (10)4 Individual): Extract AVolume (mL)）For (500-1000)）：1The proportion (recommended 500)Add 1 mL of 10000 bacteria or cellsExtract A）Processing samples, ice bath ultrasonic fragmentation (power 300 W)Ultrasound for 3 seconds, with an interval of 7 seconds, total time 3 minutes），4℃ 12000 gCentrifuge for 10 minutes， 吸取 800 μLTransfer the supernatant to a centrifuge tube and add 150 μ LExtract BMix thoroughly, 12000 g at 4 ℃Centrifuge for 10 minutesTake the supernatant and place it on ice for testing.

③Serum (slurry), culture medium and other liquid samples: Take 100 µ LAdd 1 mL of liquid sampleExtract A，4℃ 12000 gCentrifuge for 10 minutes， 吸取 800 μLTransfer the supernatant to a centrifuge tube and add 150 μ LExtract BMix thoroughly, 12000 g at 4 ℃Centrifuge for 10 minutesTake the supernatant and place it on ice for testing.

Note: When adding extraction solution B, a large amount of bubbles will be generated. It should be slowly added and mixed by blowing until no bubbles are produced. It is recommended to use a 2 mL centrifuge tube.

Creatine content detection kit (Acetyl - α - Naphthol method)Precautions during the measurement process

① The reaction should be completed within 10 minutesComplete the absorbance measurement internally. If there are a large number of samples, it is recommended to conduct the measurement in batches;

②Reagent 2 should be sealed and stored. If the color of the reagent turns dark brown, stop using it;

③Reagent 1 and reagent 2 have certain irritants, please take appropriate protective measures and avoid contact with the reagents;

④The extraction solution contains protein precipitation components, and the sample to be tested cannot be used for protein content determination; If protein concentration is used to calculate creatine content, another sample needs to be taken using PBSOr prepare physiological saline as the test sample according to the same steps, and then measure the protein concentration;

⑤If AMeasurement beyond the linear range of standard curve absorbance value: If it is higher than the highest value, it is recommended to dilute the test sample appropriately before measurement. If it is lower than the low value, it is recommended to increase the sample size appropriately before measurement, and make corresponding modifications during calculation;

⑥To ensure accurate results and avoid reagent loss, please carefully read the instructions before testing (based on the actual received instructions), confirm whether the reagent storage and preparation are sufficient, whether the operating steps are clear, and be sure to take 2-3For samples with significant expected differences, please contact the staff promptly if there are any issues during the pre testing process.

Pre purchase notice:

A. Determine the type, specifications, and quantity of reagent kits based on the indicators to be tested;

B. Confirm whether the testing equipment and conditions match;

C. Confirm whether the delivery time and receipt time meet the requirements;

★Please contact Boxbio customer service or sales specialist promptly for any questions before purchasing

Notice of Receipt:

A. Please ensure that the phone is open so that users can track the delivery status and complete the receipt based on the tracking number;

B. Please carefully inspect the item number, packaging, quantity, and appearance of the goods upon receipt (for any damage, leakage, contamination, etc.). If there are any issues with the product delivery, please contact Boxbio within 15 days;

C. After receiving the goods, please store them separately according to the temperature indicated on the product label, and use and dispose of the relevant waste correctly according to the conditions described in the instructions;

D. For product quality issues, please provide a written explanation and detailed test results for reference within 3 months from the date of receipt of the product. If you do not raise any objections to the product quality within the above period, it will be deemed that you acknowledge the product as qualified;

E. The expiration date of the BOXBIO reagent kit is 3-6 months. If the product is used unopened after the expiration date, we can exchange the product for you free of charge;

F. The express delivery costs incurred for returns and exchanges due to product quality issues will be borne by our company.

Product Customization

Boxbio provides customized services for large packaging products:

Regarding the price:The salesperson will communicate with you, inquire about the customized reagent kit you need, and negotiate the required specifications based on the sample size to be tested, and then provide you with a quotation.

Regarding packaging:Production and warehouse delivery will match the corresponding packaging according to the specifications of the reagent kit you have customized. As it is a customized product, the packaging provided may not be of regular specifications. Please be aware.

Regarding delivery time:The delivery time for customized products will be calculated based on the type and specifications of the reagent kit you have customized, and will be communicated to you by sales personnel.

Research Assistance Fund

Boxbio is committed to making scientific research easier. In order to encourage and support academic research by researchers, we have launched the "Research Assistance Fund" reward program! Any SCI article published that indicates that the product is provided by Beijing Boxbio Science&Technology Co., Ltd. can apply for the corresponding research assistance fund!

According to the journal impact factor, the reward rules are as follows:

⑴Journal impact factor: 0<IF<5, research assistance fund amount is 300 yuan;

⑵Journal impact factor: 5 ≤ IF ＜ 10, research assistance fund amount is 800 yuan;

⑶Journal impact factor: 10 ≤ IF ＜ 15, research assistance fund amount is 1500 yuan;

⑷Journal impact factor: 15 ≤ IF ＜ 20, research assistance fund amount is 2500 yuan;

⑸Journal impact factor: 20 ≤ IF, research assistance fund amount is 3500 yuan.

*Non SCI articles can apply for a beautiful gift and receive corresponding points.

Application Instructions:

⑴The article must clearly indicate that the corresponding product is sourced from Beijing Box Biotechnology Co., Ltd. or Beijing Boxbio Science ＆ Technology Co.,Ltd.；

⑵The reward is only available to the first author of the article, and co authors can only apply for one reward;

⑶The same article can only apply for research funding once;

⑷If the products cited in the literature are trial equipment, they will not participate in this activity;

⑸The interpretation rights of this event belong to Beijing Box Biotechnology Co., Ltd.

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