Mouse primary astrocytes

NegotiableUpdate on 05/06

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Overview

Immunofluorescence identification of mouse primary astrocytes as biological astrocytes, which are the largest in volume among glial cells. Many long and branching protrusions are emitted from the cell body, extending and filling between the cell body and its protrusions of nerve cells, playing a role in supporting and separating nerve cells. Fibrous astrocytes are mostly distributed in the cortex of the brain and spinal cord, with slender processes and few branches. They are found in the cytoplasm and are mostly distributed in the gray matter. The cell processes are thick and short, with many branches. There are fewer glial filaments in the cytoplasm, also known as mossy cells.

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　　Mouse primary astrocytesDetailed description:

Astrocytes are the largest type of glial cell in terms of volume. Many long and branching protrusions are emitted from the cell body, extending and filling between the cell body and its protrusions of nerve cells, playing a role in supporting and separating nerve cells.

Fibrous astrocytes are mostly distributed in the cortex of the brain and spinal cord, with slender processes and few branches. They are found in the cytoplasm and are mostly distributed in the gray matter. The cell processes are thick and short, with many branches. There are fewer glial filaments in the cytoplasm, also known as mossy cells. Under electron microscopy, the nuclei of astrocytes are missing, the cytoplasm is relatively clear, there are few free ribonucleoprotein bodies and rough endoplasmic reticulum, glycogen granules are abundant, and there are a large number of glial filaments. The protrusions of fibrous astrocytes are cylindrical in shape, while the protrusions of protoplasmic astrocytes are thin and often wrap around nerve cells and their synapses. The footplate of astrocytes is separated from endothelial cells by a substrate, and there is a half bridge granule structure at the contact between the footplate membrane and the substrate.

Important models in neuroscience research play multiple roles in the central nervous system, including supporting and maintaining neuronal survival, supplying energy, and regulating neuronal activity. Astrocytes can be classified into two subtypes based on their morphology and function: protoplasmic and fibrous. They express specific markers, such as glial fibrillary acidic protein (GFAP), which is a hallmark of mature astrocytes.

In the laboratory, researchers can isolate and culture astrocytes from the brain tissue of newborn mice using specific culture techniques. These cells exhibit a typical star shaped morphology when cultured in vitro and can be identified through techniques such as immunofluorescence staining. For example, anti GFAP antibodies can be used to label astrocytes, while other markers such as S100- β and glutamate transporter (EAAT1) can also be used to confirm cell identity.

When conducting primary culture, researchers usually choose mice within 24 hours of birth because brain tissue is more easily dissociated into single cells at this time. After dispersing brain tissue into single cells through physical methods, it can be cultured under conditions containing DMEM/F12 medium, fetal bovine serum (FBS), and trypsin. During the cultivation process, astrocytes gradually separate from other types of cells and form a monolayer in the culture dish.

　　Mouse primary astrocytesCharacteristics:

1) The organization is derived from normal brain tissue of experimental animals.

2) Cell identification: Immunofluorescence staining of glial fibrillary acidic protein (GFAP).

3) After identification, the purity of the cells was found to be higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

5) Cell growth mode: Star shaped cells, adherent culture.

Transportation mode:

Low temperature: (1) Transport in 1mL cryovials packaged with dry ice. After receiving, store in a -80 degree freezer overnight and transfer to liquid nitrogen or directly resuscitate. If you find that the dry ice has evaporated completely, the cryovial cap has fallen off, is damaged, or the cells are contaminated, please contact us immediately.

At room temperature: (2) T25 bottles of revived surviving cells will be shipped at room temperature and processed according to the procedures for cell reception upon receipt.

Bio-Safety:

1. All animal cells are considered to have potential biological hazards and must be operated in a secondary biosafety platform. Please pay attention to protection, and all waste liquids and containers that have come into contact with these cells must be sterilized before disposal.

2. It is recommended to always use protective gloves, clothing, and a face mask when reviving frozen cells.

Attention: The cryotube immersed in liquid nitrogen will leak and gradually fill with liquid nitrogen. When thawing, the conversion of liquid nitrogen into gas phase may cause the container to explode or the lid to be blown off with dangerous force, resulting in flying debris and causing personal injury.