Mouse macrophage stimulating protein (MSP) ELISA kit

Brand: ZCIBIO (ZCIBIO)

Product Name:Mouse macrophage stimulating protein (MSP) ELISA kit

Application fields:Mouse macrophage stimulating protein (MSP) ELISA kitFor scientific research experiments only, not for clinical diagnosis. Please read the instructions carefully before use

(If you need to know the product manual, please contact our customer service. We will provide you with service. The product is only for scientific research experiments and cannot be used in clinical practice. Thank you.)

Product Introduction:

Expected application: This kit is a sandwich enzyme immunoassay method for in vitro quantitative detection of concentrations in serum, plasma, tissue, cell supernatant, and related biological fluid samples.

ELISAStorage of reagent kits, unopened reagent kits: All reagents are stored as indicated on the label of the reagent bottle. Please note that upon receipt of the reagent kit, please store the TMB washing solution and termination solution at 4 ℃ as soon as possible, and store other reagents at -20 ℃. After use reagent kit: The remaining reagents should still be stored at the temperature indicated on the label of the reagent bottle. The opened enzyme-linked immunosorbent assay (ELISA) plate should be sealed with a desiccant and stored at -20 ℃ to avoid moisture.

Storage and expiration date of reagent kit:

Unopened reagent kit: All reagents are stored as indicated on the label of the reagent bottle. Please note that upon receipt of the reagent kit, please store the TMB washing solution and termination solution at 4 ℃ as soon as possible, and store other reagents at -20 ℃.

After use reagent kit: The remaining reagents should still be stored at the temperature indicated on the label of the reagent bottle. The opened enzyme-linked immunosorbent assay (ELISA) plate should be sealed with a desiccant and stored at -20 ℃ to avoid moisture

Precision:

Precision is represented by the coefficient of variation (CV) of the measured values of the sample. CV（%）=SD/mean×100

Intra batch difference: Quantitatively detect low, medium, and high value samples using the same batch of reagent kits. Each sample is measured continuously 20 times, and the average and SD values of samples with different concentrations are calculated separately.

Batch difference: Three different batches of reagent kits are selected to quantitatively measure low, medium, and high value samples. Each sample is tested 8 times using the same reagent kit, and the average and SD values of samples with different concentrations are calculated separately.

Intra batch difference: CV<10% Inter batch difference: CV<13%

Customer FAQ:

When creating a standard curve, ensure that all dilution steps have been accurately completed. Pay attention to replacing the nozzle in a timely manner when diluting. In the process of pipetting
Mix the diluent thoroughly beforehand.
How does the washing method affect my standard curve?
Washing without * will reduce the measurementSex leads to unsatisfactory standard curve results. Ensure the normal operation of the washing machine
The wells of the enzyme-linked immunosorbent assay (ELISA) plate were thoroughly washed but not dried.
How does the transfer method affect my standard curve?
Unreasonable pipetting operations can lead to high CV values and unfavorable curves.
During the process of creating standard curves, incorrect pipetting can lead to incorrect dilution, resulting in incorrect values entering the standard
In quasi curves, or generating non-linear curves. Ensure that the pipette is functioning properly. The unequal volume of liquid in each well may indicate a shift
Caused by malfunction of the liquid dispenser or improper use of the gun head.

If you also have any questions about ELISA experiments, you can contact us to discuss together. Let's learn and grow together!

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