Mouse embryonic osteoblasts

NegotiableUpdate on 05/06

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Overview

Identification of MC3T3-E1 species of mouse embryonic osteoblasts: 1) Source: parietal bone 2) Morphology: fibroblast, adherent growth 3) Content:>1x106 cells 4) Specification: T25 bottle or 1mL cryovial packaging 5) Purpose: for scientific research only

Product Details

Mouse embryonic osteoblasts MC3T3-E1Species identification
MC3T3-E1 is a mouse embryonic osteoblast precursor cell derived from the C57BL/6 strain of embryonic mouse skull. This type of cell has the ability to differentiate into osteoblasts and bone cells, and can form calcified bone tissue in vitro. The MC3T3-E1 cell line has multiple subclones and is an ideal material for studying osteoblast differentiation, osteobiology, bone metabolism, and diseases such as osteosarcoma. It is particularly suitable for studying the role of extracellular matrix (ECM) signaling pathways. The cell morphology is fibroblast like, with adherent growth. The culture conditions are 5% CO2 and 37 ℃. The commonly used medium formula is MEM α medium containing nucleosides and GlutaMAX additives, with the addition of 10% fetal bovine serum FBS. The passage cycle is about 3-4 days, the passage ratio is usually 1:3, and the frequency of fluid change is once every 2-3 days. MC3T3-E1 cells are widely used in scientific research, including osteoporosis, osteosarcoma, tooth development, and bone tissue engineering. The cell test results showed that MC3T3-E1 does not contain contamination from HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

Mouse embryonic osteoblasts MC3T3-E1
Cell identification	Species identification has been passed
Cell source	National Resource Library
Cell background	Establishment of skull from embryonic/fetal C57BL/6 mice; The differentiation into osteoblasts and the production of collagen described in the literature. This cell has multiple subclones and can serve as a good model for studying osteoblast differentiation in vitro.
Cultivation conditions:	1) Prepare MEM α (recommended awcell-0003) culture medium; High quality fetal bovine serum, 10%; Double antibody, 1%. 2) Cultivation conditions: Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃, humidity in the incubator is 70% -80%.
L Precautions:	For the passage of adherent cells, the following methods can be referred to: 1. Discard the culture supernatant and rinse the cells 1-2 times with PBS that does not contain calcium or magnesium ions. 2. Add 0.25% (w/v) trypsin 0.53 mM EDTA to culture bottles (1-2mL for T25 bottles and 2-3mL for T75 bottles), digest in a 37 ℃ incubator for 1-2 minutes (digestion time can be appropriately extended for difficult to digest cells), and then observe the digestion of cells under a microscope. If most of the cells become round and fall off, quickly take them back to the operating table, tap the culture bottle a few times, and add 3-4ml of culture medium containing 10 μ S to terminate digestion. 3. Gently mix and aspirate, centrifuge at 1000RPM for 3-5 minutes, discard the supernatant, add 1-2mL of culture medium and blow evenly. Divide the cell suspension into new T25 bottles in a ratio of 1:2, add 6-8ml of new * culture medium prepared according to the instructions to maintain the growth vitality of the cells, and subsequently subculture according to the actual situation in a ratio of 1:2~1:5. 3) Cell cryopreservation: It is recommended to freeze a batch of cell seeds during the first 3 generations of culture after receiving the cells for subsequent experimental use.
Transportation mode:	Low temperature: (1) Transport in 1mL cryovials packaged with dry ice. After receiving, store in a -80 degree freezer overnight and transfer to liquid nitrogen or directly resuscitate. If you find that the dry ice has evaporated completely, the cryovial cap has fallen off, is damaged, or the cells are contaminated, please contact us immediately. At room temperature (2), T25 bottles of revived surviving cells will be shipped at room temperature and processed according to the procedures for cell reception upon receipt.
Biosafety	1. All animal cells are considered to have potential biological hazards and must be operated in a secondary biosafety platform. Please pay attention to protection, and all waste liquids and containers that have come into contact with these cells must be sterilized before disposal. 2. It is recommended to always use protective gloves, clothing, and a face mask when reviving frozen cells. Attention: The cryotube immersed in liquid nitrogen will leak and gradually fill with liquid nitrogen. When thawing, the conversion of liquid nitrogen into gas phase may cause the container to explode or the lid to be blown off with dangerous force, resulting in flying debris and causing personal injury.