Mouse cardiac troponin I (cTn-I) ELISA kit

1、 Parameter specifications

:Mouse cardiac troponin I (cTn-I) ELISA kit-Quantitative

:1-3sky(In stock)

: Qualitative and Quantitative Analysis of Tested Samples

:96T/48T(two specifications)

【 Detection Method 】: Enzyme Immunoassay/Enzyme linked immunosorbent assay(ELISA)

:2-8℃

: Liquid boxed packaging

:(Tax and shipping included, we provide full coverage throughout the processELISAExperimental Technical Guidance andELISAFree testing of reagent kits)

: Industry price advantage, reliable quality, and guaranteed replacement and refund in case of objective quality issues

【 Sample to be tested 】: body fluids, serum, plasma, cell culture supernatant, urine, tissue homogenate, atrial water standard, etc

2、 Specimen requirements

1. Serum: Warm blood naturally coagulates10-20After minutes, centrifuge20minutes(2000-3000turn/part)Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.

2. Plasma: Select according to the requirements of the specimenEDTASodium citrate or other anticoagulants, mixed together10-20After minutes, centrifuge20minutes(2000-3000turn/part)Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.

3. Urine: collected in sterile tubes. centrifugation20minutes(2000-3000turn/part)Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again. Follow this procedure for pleural effusion, ascites, and cerebrospinal fluid.

4. Cell culture supernatant: When detecting secreted components, collect them using sterile tubes. centrifugation20minutes(2000-3000turn/part)Carefully collect the supernatant.

5. Tissue specimen: After cutting the specimen, weigh it. Add a certain amountPBS，PH7.4Quickly freeze and store in liquid nitrogen for future use. The specimen remains intact even after melting2-8The temperature is ℃. Add a certain amountPBS(PH7.4)Alternatively, use tissue protein extraction reagents to homogenize the specimen manually or with a homogenizer. centrifugation20minutes(2000-3000turn/part)Carefully collect the supernatant. After packaging, one portion is to be tested, and the rest is to be frozen for future use.

3、 Question and Answer

ElisaAnalysis of negative and positive controls in the reagent kit

ElisaNegative and positive controls of the reagent kit: If the negative and positive controls have correct results, a rough judgment can be made on the results of the reagent kit operation.

Blank control: YesELISAIn terms of reagents, blank control is actually a control without adding samples or enzymes. The blank control mainly measures the absorbance of the system. Blank control is particularly important in single wavelength measurements

Our company represents thousands of typesELISAReagent kits, tens of thousands of antibodies, and nearly ten thousand products represented, covering various fields of life science research such as molecular biology, molecular immunology, and cell biology. To save your time, we can also provide proxy testing services.

4、 Precautions

1. Due to the sensitivity of substrate chromogens to light, they should be stored away from light.

2. Before detecting absorbance, the enzyme-linked immunosorbent assay (ELISA) reader should be turned on and preheated30minThe above.

3. The incubation time should strictly follow the instructions on the reagent kit.

4. Double well experiments should be conducted as much as possible to ensure the accuracy of data and reflect the precision of the reagent kit.

5. When there are doubts about the results of the sample, other testing methods need to be used for verification.

5Mouse cardiac troponin I (cTn-I) ELISA kit-QuantitativeProxy testing service

Customers can receive samples by express delivery, and customers in Shanghai can enjoy our on-site sampling service for sample testing.

The newly released reagent kit on our company's website can provide proxy testing services.

The reagent kits not yet listed on our company's website can be developed and tested on our behalf, and related information such as costs can be consulted by phone.