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Shanghai Hengyuan Biotechnology Co., Ltd
Mouse Adenosine Deaminase (ADA) Kit - Activity
NegotiableUpdate on 05/06
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Overview
Hengyuan Biochemical Reagent Kit has a wide range of applications, including enzymes, oxidative stress, iron death, liver function, kidney function, glycolysis, inorganic salt ions, lipid metabolism, plant stress resistance, etc. There are a variety of sample types for detection, and the main detection instruments are enzyme-linked immunosorbent assay (ELISA), spectrophotometer, and fluorescence ELISA. We will provide customers with efficient and professional biochemical testing services and experimental technology solutions with a high sense of responsibility and excellent product quality. Rich experience, excellent quality, sufficient inventory, and accurate delivery time all guarantee the professional and high-quality service we provide to you.
Product Details
| Chinese name | Mouse Adenosine Deaminase (ADA) Kit - Activity |
| specification | 48 samples |
| Item Number | P-870-SH |
| price | Negotiable |
| Sample Type | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant, etc |
| Testing Scope | Refer to the instruction manual for details |
| detection method | colorimetry |
| delivery time | 3-5 days |
| Instruction manual | Consult our sales representative to obtain |
| literature | Consult our sales representative to obtain |
note meaning ：Before formal testing, it is necessary to take2-3Make predictions based on samples with significant expected differences
Research significance:
Determine the total antioxidant level composed of various antioxidant substances and antioxidant enzymes in the object. In biological, medical, and pharmaceutical research, various body fluids such as plasma, serum, saliva, urine, cell or tissue lysates, plant or herbal extracts, and various antioxidants are often detected(antioxidant)The total antioxidant capacity of the solution.
Measurement principle:
DPPH·For stable free radicals Dissolve in polar solvents such as methanol and ethanol515nmThere is maximum absorption at this location. to, toward, at, facingDPPH·When antioxidants are added to the solution, a decolorization reaction occurs, so changes in absorbance can be used toTroloxQuantify the antioxidant capacity of antioxidant substances as a control system.
Self provided experimental supplies:
Constant temperature water bath, low-temperature centrifuge, enzyme-linked immunosorbent assay reader96 Orifice plate and distilled water.
Reagent composition and preparation:
Extraction solution: liquid120mL×1 Bottle, pre cool before use.
Reagent 1: Liquid45mL×1 Bottle, store away from light.
Preparation of samples:
(1) Liquid samples such as serum, plasma, saliva, or urine
Plasma (heparin or sodium citrate can be used for anticoagulation during preparation, but should not be used)EDTAAnticoagulation)4℃，5000rpmcentrifugation10minTake the supernatant for testing. Serum, saliva, or urine samples can be directly used for measurement, or they can be-80Freezing at ℃ (should not exceed)30 d）Measure again later.
(2) Organize samples
According to organizational quality（g）Volume of extraction solution(mL)for 1：5~10The proportion of (recommended to be around)0.1gOrganize, join1mLExtract the liquid and homogenize it in an ice bath, then10000g，4℃ centrifugation10minTake the supernatant and place it on ice for testing.
(3) Cell samples
According to the number of cells（104 Individual: volume of extraction solution（mL）For500~1000：1 The proportion (suggested) 500 Ten thousand cells added1mLExtraction solution), ice bath ultrasonic fragmentation (power)200W, Ultrasound3s, interval10sRepeat, repeat 30 Secondary);10000g，4℃ centrifugation10minTake the supernatant and place it on ice for testing.
Operation steps:
1Preheating of ELISA reader30minAdjust the wavelength to515nm.
2Operation table (in)EPTube reaction)
Blank tube |
Measurement tube |
|
Extraction solution（ML） |
20 |
|
Sample（ML） |
20 |
|
Reagent 1（ML） |
380 |
380 |
Mix thoroughly and avoid light reaction at room temperature 20min， 取 200MLto 96 Orifice plate measurement 515nmAbsorbance value,△A=Ablank-Adetermination | ||
AttentionBlank tubes only need to be measured once, ifAdeterminationless than 0.2It needs to be diluted with extraction solution and tested.
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