Monkey Valley Amino Acid Decarboxylase Antibody (GADAb) ELISA Kit

For scientific research purposes only, this kit is used to determine the content of glutamic acid decarboxylase antibodies (GADAb) in monkey serum, plasma, and related liquid samples.

【 Detection Principle 】

This kit uses the double antigen sandwich method to determine the level of monkey glutamic acid decarboxylase antibody (GADAb) in the specimen. Coat the microporous plate with purified antigen to prepare solid-phase antigen. Add glutamic acid decarboxylase antibody (GADAb) to the micropores coated with antigen in sequence, and then bind with HRP labeled antigen to form an antigen antibody enzyme labeled antigen complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with the glutamic acid decarboxylase antibody (GADAb) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of monkey glutamic acid decarboxylase antibody (GADAb) in the sample using a standard curve.

1、 物理性能

The liquid components of the reagent kit should be clear and transparent, without precipitation or flocculent substances. Microporous aluminum foil bags should be vacuum packed without damage or air leakage (if there is air leakage, it does not affect use).

2. Linear dose-response curve

The correlation coefficient r value of the standard dose response curve is greater than or equal to 0.9900.

3. Detection range

0.5ng/ml - 26ng/ml 。

4. Sensitivity

The lowest detectable dose is less than 0.125 ng/ml.

5. Precision

Precision is represented by the coefficient of variation (CV) of the measured values of the sample. CV（%） = SD/mean×100

Intra batch difference: Quantitatively detect low, medium, and high value samples using the same batch of reagent kits. Each sample is measured continuously 20 times, and the average and SD values of samples with different concentrations are calculated separately.

Batch difference: Three different batches of reagent kits are selected to quantitatively determine low, medium, and high value samples. Each sample is tested 10 times using the same reagent kit, and the average and SD values of samples with different concentrations are calculated separately.

Within batch deviation: CV<5.1%

Batch difference: CV<6.5%