Model of macroporous adsorption resin for protein extraction and purification

Model of macroporous adsorption resin for protein extraction and purificationMacroporous adsorption resin is composed of styrene and propionate as monomers, ethylene benzene as crosslinking agent, toluene and xylene as pore forming agents, which crosslink and polymerize with each other to form a porous skeleton structure. Resin is generally white spherical particles and is a type of cross-linked polymer containing ion exchange groups. Its physical and chemical properties are stable, insoluble in acids, bases, and organic solvents, and not affected by inorganic salts and strong ion low molecular compounds.

The adsorption effect of macroporous resin relies on the van der Waals attraction between it and the adsorbed molecules (adsorbate). Through its huge specific surface area for physical adsorption, organic compounds can be separated by certain solvents based on their adsorption force and molecular weight, achieving different purposes such as separation, purification, impurity removal, and concentration. Macroporous adsorption resin is a separate material that combines the principles of adsorption and selectivity. The adsorption essence of macroporous adsorption resin is a surface adsorption phenomenon caused by highly dispersed objects or uneven surface molecule effects. This adsorption performance is due to van der Waals attraction or the generation of hydrogen bonds; Due to the porous structure of macroporous adsorption resin, it has a selective effect on substances with different molecular sizes. Based on the principle of adsorption and selection mentioned above, organic compounds are eluted with certain solvents on macroporous adsorption resins according to their different adsorption forces and molecular weights to achieve their respective goals.

Model of macroporous adsorption resin for protein extraction and purificationThe wide adsorption conditions and the selection of adsorption conditions directly affect the quality of the adsorption process of macroporous adsorption resin. Therefore, various factors should be comprehensively considered throughout the entire process to confirm the optimal adsorption and desorption conditions. There are many factors that affect resin adsorption, mainly including the properties of the separated components (polarity and molecular size, etc.), the properties of the loading solvent (solubility of the solvent in the components, salt concentration, and pH value), the concentration of the loading solution, and the flow rate of the adsorbed water.