MoFlo Astrios EQ Ultra High Speed Flow Sorting System

MoFlo Astrios EQ Ultra High Speed Flow Sorting SystemSimplify your most complex sorting tasks with 7 independent laser controls and six air excited sorting channels, while reducing operational complexity. This series of instruments is simple, flexible, and has an intuitive interface, allowing you to witness advantages that other sorting systems cannot match.

The newly launched flagship version MoFlo Astrios EQs and dual forward EQ sorter fully utilize the stable flow control and electronic design of the original MoFlo Astrios. They not only have a wide range of laser selection and six channel sorting functions, but also add enhanced scattered light (eFSC; under review) technology that can detect submicron particles, thereby expanding the sorting function. Seven small holes separate up to seven laser spaces to complete complex multi-color sorting tasks. The leveling multi laser beam shaper (MLSO) generates an evaluation beam distribution, shortens calibration time, and improves optical stability. Two micrometer bases control the calibration effect of all seven lasers, simplifying sorting preparation work and shortening setup time. Advanced software components enhance instrument functionality. The operation of the touch screen control panel has been optimized for the workflow of the experimental center, making daily settings very convenient and suitable for use with optional integrated biosafety cabinets.

MoFlo Astrios EQ Ultra High Speed Flow Sorting Systemoptical device

A configurable system with 7 lasers and 7 small holes: 355nm, 405nm, 488nm, 532nm, 560nm, 592nm, 645nm (wavelength is the standard wavelength and can be changed).

A single multi laser beam shaper (MLSO) is used for optically coupled lasers.

The flat top beam distribution on the fiber coupler improves beam stability and reduces calibration difficulty.

Up to 7 lasers can be calibrated in two stages to speed up sorting settings.

software

IntelliSort II - a sorting instrument with droplet free liquid delay determination and detection function.

Touch screen control panel for instrument settings and sorting monitoring (only for operation and cross training).

The laser automatically turns on and the jet increases automatically.

Single button automatic quality control, tracked through strengthened troubleshooting guidance.

Summit software version 6.2.4 (or higher).

performance

Multiple collection devices form a 6-channel sorting system (all sorting outputs are temperature controlled through a water bath).

Mixed mode sorting: Individual sorting modes (enrichment, purification, single-cell) can be set for each sorting stream. The cells discarded by the software can be collected for further analysis or re sorted.

Index based sorting uses intuitive cell maps.

Support 1536 orifice plate sorting, with reproducible results (as the jet remains stable).

A sorting instrument with a validated performance of 70000 sorting speeds per second.

Electronic components and software

Electronic components can simultaneously detect 44 parameters.

32-bit processing and 100MHz sampling speed.

The extremely short electronic dead time means that the hardware dropout rate is very low.

Verify performance with a collection rate of 100000 events per second.

PMT has a dynamic range of 5 orders of magnitude.

Up to 2 FSC channels, including user selectable FSC baffles and additional optional SSC channels (capable of detecting wavelengths greater than or equal to 405nm).

1 billion event. FCS files have no parameter restrictions

生物安全性

The customized Baker SterilGARD BSL2 biosafety cabinet provides biological protection without obstructing the sorter jet and optical display.

The integrated configuration of the biological cabinet is an aerosol removal system, which controls the direct removal of aerosols from the sorting chamber of the sorter to the HEPA shell.

The experience test of the biological cabinet meets the NSF-49 standard.

The optional Aerosol Exhaust System (AES) discharges particles from three (3) chambers, and its performance complies with ISAC guidelines in the absence of a safety cabinet.

The quick release nozzle design facilitates nozzle replacement.

The sorting room has no hard edges, which is conducive to aerosol discharge and cleaning.

The CyClone board arm is equipped with an arc brush, which can seal the sorting chamber.