Microelectrode puller (needle puller)

The P-97 programmable horizontal microelectrode puller from Sutter Corporation in the United States is commonly used for the fabrication of various glass, quartz, and other microelectrodes. As commonly used microelectrodes in experiments, it is widely used in electrophysiology and cell injection experiments. Sutter Corporation's P-97 programmable horizontal microelectrode puller has been identified as the standard equipment for electrophysiology and cell injection laboratories.

Microelectrode puller (needle puller)performance metrics：

The P-97 needle puller produces smooth and neat electrodes that do not require grinding.

The P-97 microelectrode puller is a Flaming/Brown type microelectrode, patch clamp electrode, and microinjection needle puller. Adhering to many advantages of the P-87, the P-97 further enhances the design of mechanical, electrical, and software aspects. The drawing process is easier to control and has a higher level of repeatability. The P-97 integrates mature programmable microprocessors into reliable mechanical systems. Programmable control of drawing parameters enables researchers to design electrodes for specific applications across a wide range of glass compositions and sizes.

Many new mechanical features were integrated into the design of P-97. The most obvious one is the cavity wrapped around the heating wire. The humidity control chamber is designed to minimize the impact of humidity changes on repeatability in the drawn electrode. The power has increased by 25% compared to the previous version, making it suitable for larger heating wires, larger diameter glass tubes, and multi tube glass. The redesign of the metal clamp that holds onto the heating wire minimizes residual heat. The air supply switch provides external cooling for large diameter and multi tube glass. Add a spring clamping mechanism to facilitate the fixation of glass electrodes. Increasing vacuum fluorescence display facilitates observation from all directions.

The new software improves the display of heating time, which helps with program expansion and fault diagnosis. Up to 100 more programs can be written and stored in memory, making the P-97 more useful for many users. Now programs can set write protection; This added protection prevents programs from being modified and accidentally deleted. After the program is written, the programming and editing dates are also stored in it. Atmospheric pressure is also a programming parameter.

The speed at which the glass softens and begins to separate under a fixed tension is measured. The initial drawing speed stops increasing due to the viscosity of the glass, followed by the temperature of the glass. Adjustable speed allows for precise selection of glass temperature to the critical point of excessive tension. The drawing machine uses the principle of rapid cooling of heating wires to create a special short cone for the pointed and fine electrodes, resulting in electrodes that are particularly hard and suitable for grinding at oblique angles. Another advantage of a short cone is that the connected dual tube electrodes reduce resistance and capacitance.