Microbial pyruvate kinase (PK) ELISA kit

Microbial pyruvate kinase (PK) ELISA kit
English name: Microbialpyruvatekinase (PK) ELISAkit
Brand: Ruichuang
Item number: RC-W2127S
Specification: 96T/48T
This product is suitable for scientific research experiments but not for clinical use
This kit is used for quantitative detection of serum, plasma, tissue homogenate, and related liquid samples in vitro.
Samples: serum, plasma, tissue fluid, tissue homogenate, etc
Tianjin Ruichuang Biotechnology Co., Ltd. specializes in ELISA kits, ELISA reagents, culture media, serum, antibodies, proteases, and peptides

1. Principle: Immunoassay is a method that utilizes the high selectivity and specificity recognition and binding principle between specific antibodies and antigens or haptens to analyze and determine the target antibody or antigen. Enzyme linked immunosorbent assay (ELISA) is a type of immunoassay that consists of three parts: immune recognition, signal output, and data processing.

Step 2: Immunorecognition involves coating a polystyrene 96 well plate with antibodies, and then using the antibodies to recognize the antigen to be tested (usually protein biomarkers of diseases, viruses, bacteria, etc.). The antigen is adsorbed onto the surface of the 96 well plate from a complex test solution. Then, the recognition signal is output directly or indirectly using antibodies labeled with horseradish peroxidase (HRP), fluorescence, or radioactivity. Then, the concentration of the target antigen in the test sample is calculated using information such as signal strength and concentration gradient of the standard sample.

3. Classification: According to the different methods of immune recognition and signal output, ELISA can be divided into double antibody sandwich method, direct immune competition method, non direct immune competition method, and so on. The double antibody sandwich method is commonly used in commercial applications.

Microbial pyruvate kinase (PK) ELISA kitNotes:

1. All operation processes are generally handled with a blowdown gun. During the operation, the blowdown gun is set to two gears and one gear is set to prevent the generation of bubbles. During operation, it is necessary to ensure that the gun head is inserted tightly at every step. Choosing the matching gun head is particularly important, as it is easy for the gun head to fall off and affect the experimental results. In addition, it is important to ensure that the gun head page is level at each step to avoid the gun head becoming loose without realizing it.

During the process of handling diluted standard samples, it is necessary to tap on the vortex analyzer to ensure even mixing, but the oscillation process should not be too long to prevent protein degradation and denaturation

3. The dilution concentration of secondary antibodies plays a crucial role in the color development effect. Each time a new secondary antibody is selected or a secondary antibody that has been pre packaged in a -80 degree refrigerator is selected, a pre experiment is conducted to set different secondary antibody concentrations and color development times, in order to determine the corresponding conditions for large-scale cell screening.

4. The incubation time of ELISA for the same cell line should be consistent to reduce the influence of irrelevant variables.

5. The storage of various antibodies is in a -80 degree freezer, and it is necessary to reduce the number of freeze-thaw cycles. Proteins are more sensitive, and with more freeze-thaw cycles, protein activity will decrease.

When diluting the sample, it is necessary to ensure that it is mixed evenly, and the tip of the gun should be blown the same number of times below the liquid level to prevent the occurrence of bubbles.

In the process of analyzing data, it is generally necessary to have at least 5 points, otherwise the data will not have corresponding persuasiveness.

After taking the sample from the 8.96 well plate, cover it and add DPBS diluent in advance to prevent sample evaporation and inaccurate concentration due to prolonged time.
Disclaimers
1. The reagent kit is for research purposes only and should not be used for clinical or human experiments. Any consequences arising from this shall be borne by the experimenter, and our company shall not be held responsible.
2. Strictly follow the instructions for operation. If the experimenter violates the instructions, the consequences shall be borne by the experimenter.
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