Microbial 6-phosphofructokinase 1 (6PFK-1) ELISA kit

Microbial 6-phosphofructokinase 1 (6PFK-1) ELISA kit

English name: Microbial6-phosphatefructosekinase1 (6PFK-1) ELISAkit
Brand: Ruichuang
Item number: RC-W2128S
Specification: 96T/48T
Marker: HRP marker
This product is suitable for scientific research experiments and not for clinical use.
This kit is used for quantitative detection of serum, plasma, tissue homogenate, and related liquid samples in vitro.

Detectable samples: serum, plasma, tissue fluid, tissue homogenate, etc
Tianjin Ruichuang Biotechnology Co., Ltd. specializes in ELISA kits, ELISA reagents, culture media, antibodies, serum, proteases, and peptides
Microbial 6-phosphofructokinase 1 (6PFK-1) ELISA kit
Detection principle:
Adopting double antibody sandwich enzyme-linked immunosorbent assay. Add the specimen, standard sample, and HRP labeled detection antibody to the pre packaged captured antibody packaging micropores at once, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to yellow under the action of acid. The depth of color is positively correlated with the reagents in the sample. Measure the absorbance at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.

Notes:
1. Incubate strictly according to the prescribed time and temperature to ensure accurate results, and all reagents must reach room temperature of 20-25 ℃ before use. Immediately refrigerate and store the reagents after use.
Incorrect board washing can lead to inaccurate results.
3. Eliminate residual liquid and fingerprints on the bottom plate, otherwise it will affect the OD value.
4. The substrate color solution should be colorless or very light in color, and the substrate solution that has turned blue cannot be used.
Disclaimers
1. The reagent kit is for research purposes only and should not be used for clinical or human experiments. Any consequences arising from this shall be borne by the experimenter, and our company shall not be held responsible.
2. Strictly follow the instructions for operation. If the experimenter violates the instructions, the consequences shall be borne by the experimenter.
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