Microbial Desaturase (DT) ELISA Kit

Before the experiment, equilibrate all reagents to room temperature, including the sample and TMB (very important).

2. It is strongly recommended that teachers perform double wells on each sample, including standard samples, during the operation (please be sure to do so), as this can: calculate the average value, ensure the accuracy of the experimental results, evaluate the performance of the reagent kit, evaluate their own operation, and solve the problem of skipping wells

3. To ensure the accuracy of the teacher's ELISA experiment results, please refer to several important operating precautions: If the following statements are inconsistent with the instructions, please follow the requirements of the corresponding instructions for the experiment.

1) Standard dissolution: The standard dissolution should be strictly operated according to the instructions. It is recommended that the teacher dilute the standard in an EP tube.

2) Incubation: When incubating according to the instructions, it is important to use a microplate shaker to oscillate, which is beneficial for the full binding of antigens and antibodies.

3) Washing the board: It is recommended to follow the instructions and soak the washing solution for at least 30 seconds each time, repeating 6 times. If the washing time is too short or the number of times is too few, it will lead to poor experimental results. Extending the washing time and increasing the washing frequency can make the background cleaner.

4) How to control the color development of the standard curve (only for the double antibody sandwich ELISA kit) within an empirical range of 5.30 minutes? For each specific experiment, the approximate color development time can be determined based on the following conditions:

① Visual observation: When there is a light blue color in the S5 hole of the standard curve and no obvious blue color in the Blank hole, it can be terminated.

② Instrument judgment: At a wavelength of around 630 nm, when the OD value of the S1 hole of the ac standard curve reaches 0.5-0.7, the OD value of the S5 hole reaches 0.05-0.08, and the OD value of the Blank hole is less than 0.05, it can be terminated.

③ The high-sensitivity series reagent kit requires strict control of the color development time due to its higher sensitivity, and can shorten the color development time appropriately compared to ordinary reagent kits.

Disclaimers
1. The reagent kit is for research purposes only and should not be used for clinical or human experiments. Any consequences arising from this shall be borne by the experimenter, and our company shall not be held responsible.
2. Strictly follow the instructions for operation. If the experimenter violates the instructions, the consequences shall be borne by the experimenter.
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