Manual mechanical simple mouse tail vein fixator imaging device

Manual mechanical simple mouse tail vein fixator imaging device

Manual mechanical simple mouse tail vein fixator imaging device

1. The use of quick loading mouse tube:

Quick loading of mouse tubes is a rear tail loading method. Suitable tubes of 20g and 40g are used according to the weight of the experimental mouse. The plug is removed and the front end of the tube can smoothly pull the mouse into the tube. To avoid the mouse rolling its body inside the tube, a front plug can be used to block it inside. The hole of the front plug is designed according to the physiological curve of the mouse skull, which can effectively control the mouse's head without affecting normal breathing. At the same time, it can also allow the mouse's forelimbs to find a comfortable position. The fixed position of the plug should be based on the animal's inability to move freely without affecting lung expansion and normal breathing. It can be observed and adjusted through transparent organic glass.

2. Preparation work:

Connect the power supply of the instrument and the foot operated switch. Use the foot operated or red button to control the pressure bar switch. Note: The foot switch cannot be used in conjunction with the red pressure lever button. Place the installed mouse stabilizer on the slot of the upper panel of the host, and place the mouse tail through the open baffle in the groove of the lower plate of the mouse tail compression plate. Turn on the transparent light switch (green button), the transparent light will turn on, adjust the position of the magnifying glass to observe whether the blood vessels in the mouse tail are connected. When reading, you can adjust the yellow brightness knob according to the situation of the yellow light transmission through the blood vessels to make the blood vessels clearest (changes in environmental brightness can affect the appearance of blood vessels, it is recommended to operate indoors in a dark environment and avoid direct sunlight).

3. Adjustment of injection position:

No matter how large the mouse is used, the needle should be injected from the lower one-third of the tail of the mouse, and gradually injected from bottom to top through a two-dimensional regulator to avoid leakage from the injected needle hole. Due to the different sizes and tail lengths of animal strains used, it is necessary to adjust the injection position