Malachite Green Rapid Detection Kit/Paper Card

Detection Principle

The rapid detection card for malachite green applies the principle of competitive inhibition immunoassay. During the flow of malachite green in the sample, it binds to a specific monoclonal antibody labeled with colloidal gold, inhibiting the binding of the antibody to the malachite green BSA conjugate on the NC membrane detection line (T line), resulting in a change in the color of the detection line.

Sample collection and pre-processing

During the experiment, a red marker pen was used for marking. The black marker pen contains crystal violet, which can contaminate the fish and shrimp samples. Pre treatment method for fish and shrimp samples:

(1) Homogenize tissue samples using a homogenizer;

(2) Weigh 1.00.05g of homogeneous material into a 10ml new polystyrene centrifuge tube, add 1ml of deionized water, vortex for 2 minutes, add 100ml of malachite green extractant 1, and then add 1 bottle of malachite green extractant 2, vortex for 2 minutes, above 3000g, centrifuge at room temperature (20-25 ℃) for 5 minutes;

(3) Transfer 1ml of the supernatant to a 10ml new polystyrene centrifuge tube, add 50 μ l of malachite green oxidant, vortex for 15 seconds, and dry under nitrogen or air flow in a 50-60 ℃ water bath;

(4) Add 0.3ml of the sample complex solution and vortex it for 3 minutes, then take 100ml for analysis.

Operation steps of peacock green rapid detection card

(1) Remove the reagent bucket from the original packaging, open it and take out the required number of microporous reagents and test paper cards, and mark them properly. Please use it as soon as possible within 60 minutes. After removing the required microporous reagent, please immediately cover the reagent barrel lid to prevent moisture.

(2) Use a micropipette to aspirate 100 l of the test sample solution into the micropores, slowly aspirate and mix thoroughly with the reagents in the micropores.

(3) After incubating at room temperature (20-25 ℃) for 3 minutes, take about 100ul of the mixed solution and drop it vertically into the sample well (S well).

(4) Start timing when the liquid flows, react for 5 minutes, and determine the result according to the schematic diagram. Other times are considered invalid.