MB Mycoplasma detection kit qPCR (classical method)

MB Mycoplasma detection kit qPCR (classical method)

Mycoplasma fluorescence quantificationPCR法 （qPCR）：It's in the usual wayOn the basis of PCR, probes targeting mycoplasma specific conserved sequences are fluorescently labeled to make the PCR amplified products fluorescent. Real time monitoring of DNA amplification reactions is carried out using changes in fluorescence signals and supporting software, abbreviated as qPCR.

Advantages:

① High sensitivity and strong specificity.

② Short time, results will be available in 2-3 hours

③ The test results can be quantified

④ Easy to operate

Disadvantages:

① For samples that have undergone mycoplasma inactivation treatment, false positives may occur in PCR results due to the presence of mycoplasma DNA. (Cultivation method can be used for supplementary verification)

② The quality of reagent kits produced by different manufacturers varies greatly (due to differences in primers and internal design), and careful selection is necessary. It is recommended to use them under the guidance of professional personnel as much as possible.

MB Mycoplasma detection kit qPCR (classical method)Introduction

GermanyThe Mycoplasma qPCR detection kit produced by MB company (all using probe method for detection), based on subtle differences in operating procedures, There are two types: 'Classic Method' and 'One Step Method'.

These two types of reagent kits have the following advantages compared to similar products from other manufacturers around the world:

① The classic qPCR kit follows the European Pharmacopoeia process requirements

② High specificity, a single test can cover all possible mycoplasma species that may infect cells (including 9 mycoplasma species specified in the European Pharmacopoeia), and based on sequence consistency, at least 107 mycoplasma species can be detected. Easy to operate and easy to observe (using MB reagent kit, all mycoplasma species listed in the table below are positive, while other microorganisms or eukaryotic cells are negative, with no cross reactivity).

③ High sensitivity, from MB companyMycoplasmaQPCR detection kitThe detection limit of the classical method can reach ≤ 10CFU/ml.

MB Company'sMycoplasmaQPCR detection kitone-step methodThe detection limit is ≥ 50 genomes/reaction.

④ MB company has corresponding standard products for sensitivity testing of 10CFU/100CFU,Facilitating methodological validation.

⑤MB CompanyThere are corresponding matching mycoplasma genomic DNA and bacterial genomic DNA,Facilitating specificity verification.

⑥MB CompanyThere are corresponding absolute quantitative standards for Mycoplasma, which are convenient for final quantitative detection.