MB Fluorescent Quantitative PCR Detection Kit (qPCR)

NegotiableUpdate on 05/06

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Overview

MB Fluorescent Quantitative PCR Detection Kit (qPCR): It is a real-time monitoring of DNA amplification reaction using fluorescence signal changes and supporting software, based on conventional PCR, by fluorescently labeling the "probe for mycoplasma specific conserved sequence" to make the PCR amplified product fluorescent.

Product Details

MB Fluorescent Quantitative PCR Detection Kit (qPCR)

MycoplasmaIntroduction to qPCR detection kit

GermanyThe Mycoplasma qPCR detection kit produced by MB company (all using probe method for detection), based on subtle differences in operating procedures, There are two types: 'Classic Method' and 'One Step Method'.

These two types of reagent kits have the following advantages compared to similar products from other manufacturers around the world:

① The classic qPCR kit follows the European Pharmacopoeia process requirements

② High specificity, a single test can cover all possible mycoplasma species that may infect cells (including 9 mycoplasma species specified in the European Pharmacopoeia), and based on sequence consistency, at least 107 mycoplasma species can be detected. Easy to operate and easy to observe (using MB reagent kit, all mycoplasma species listed in the table below are positive, while other microorganisms or eukaryotic cells are negative, with no cross reactivity).

MB荧光定量PCR检测试剂盒（qpcr）

③ High sensitivity, from MB companyMycoplasmaQPCR detection kitThe classic detection limit can reach ≤ 10CFU/ml.

MB荧光定量PCR检测试剂盒（qpcr）

MB Company'sMycoplasmaQPCR detection kitone-step methodThe detection limit is ≥ 50 genomes/reaction.

④ MB company has corresponding standard products for sensitivity testing of 10CFU/100CFU,Facilitating methodological validation.

⑤MB CompanyThere are corresponding matching mycoplasma genomic DNA and bacterial genomic DNA,Facilitating specificity verification.

⑥MB CompanyThere are corresponding absolute quantitative standards for Mycoplasma, which are convenient for final quantitative detection.

MycoplasmaQPCR detection kitcharacteristic

1. The internal control control control is independent packaging, following the operating procedures of the European Pharmacopoeia and the Japanese Pharmacopoeia.

2High specificity, one test can cover all common mycoplasma species that are susceptible to infection in cells (including the 9 mycoplasma species specified in the European Pharmacopoeia). No cross reactivity with bacteria and eukaryotic DNA.

3.High sensitivity, detection limit can reach≤10CFU/ml。

4. Corresponding matching mycoplasma genomic DNA and bacterial genomic DNA for specificity verification.

5. There are corresponding absolute quantitative standards for Mycoplasma, which are convenient for final quantitative detection.