Laboratory short wave ultraviolet cross-linking sterilization box

Laboratory short wave ultraviolet cross-linking sterilization box

LUYOR UV cross-linking instrument UCL-3500 laboratory short wave ultraviolet sterilization box UV Crosslinkers

There are three modes to choose from: automatic cross-linking, time, and energy.

Performance of the Purple Diplomacy Instrument UCL-3500:

Microprocessor control: programmable microprocessor monitors the intensity of ultraviolet radiation in real-time. When the set energy is reached, the irradiation will immediately stop. There will be no energy impact caused by the decrease in UV intensity due to the aging of the lamp tube.

Uniformity: The irradiation chamber adopts stainless steel mirror and orange optical reflector plates. The ultraviolet sensor unit is located above the irradiation chamber and is equipped with a quartz glass protective cover to ensure that the sensor is not contaminated and durable for a long time.

Easy to use: Multiple modes such as time mode, energy mode, preset mode, etc. can be selected for operation.

Quick: By default, it enters the pre shutdown operation setting program for one click operation, which is convenient and fast.

Beep reminder: Cross link is completed, beep sound reminder, no operation required, automatically enters standby mode.

Easy to operate: microprocessor control, pre-set common programs, easy to operate!

Uniform lighting: The combination of mirror emitting plate and orange reflective plate ensures uniform light intensity throughout the irradiation room!

Three year warranty: Provide free replacement in case of malfunction within one year and free warranty within three years!

The main applications of the Purple Diplomacy Instrument UCL-3500 are:

UV induced mutation

Eliminate PCR contamination and UV sterilization

Screening for RecA mutations in Escherichia coli

Membrane cross-linking of nucleic acids

Biomolecular cross-linking

DNA cleavage in agarose gel

Technical data:

UCL-3500 UV wavelength: 254 nanometers

UCL-3500M UV wavelength: 302 nanometers

UCL-3500L UV wavelength: 365 nanometers

UV tube: 6 x 15 watts

Programming: 9 preset radiation energy values

Energy: 0.000 to 9.999 joules or 0.00 to 99.99 joules

Time: 00.00 to 99.59 minutes or 000.0 to 599.5 minutes

Temperature: 15 ℃ -35 ℃

Humidity: 70% without condensation

Shell surface temperature: ≤ 30 degrees Celsius

Start time:<1 second

External dimensions (length x width x height): 55cm x 35cm x 33cm

Internal dimensions (length x width x height): 46cm x 33cm x 16cm

Weight: 11 kilograms

Power supply: 100-115V AC&230V AC 50/60Hz

Qualification certification: CE

UCL-3500 Purple Diplomacy Instrument Used for CLIP seq Experiment:

The UCL-3500 ultraviolet radiation analyzer is a 254nm shortwave ultraviolet radiation analyzer, which is equipped with six 15W 254nm ultraviolet light tubes and an aluminum alloy irradiation chamber. The irradiation chamber has a large area and uniform ultraviolet radiation, which meets various ultraviolet irradiation experiments. It is a tool for Northern, Southern, Slot or Dot imprinting film fixation and CLIP seq experiments.

CLIP-seq， Also known as HITS-CLIP, which stands for cross-linking immunoprecipitation and high-throughput sequencing, is a revolutionary technology that reveals the interactions between RNA molecules and RNA binding proteins at the whole genome level.

The main principle of CLIP seq is based on the coupling between RNA molecules and RNA binding proteins under 254nm ultraviolet irradiation. After the RNA protein complex is precipitated with RNA binding protein specific antibodies, the RNA fragments are recovered, and high-throughput sequencing is performed on these molecules through the addition of adapters, RT-PCR, and other steps. Then, bioinformatics analysis, processing, and summarization are carried out to uncover their specific patterns, thereby revealing the regulatory role of RNA binding proteins and RNA molecules and their significance for life.

CLIP seq UV irradiation energy is set to:

Turn on the power of the UCL-3500 Purple Diplomacy Instrument.

Place the cells to be irradiated into the irradiation chamber of the ultraviolet radiation analyzer.

Press the program button to select energy mode, and the green indicator light for energy mode will turn on.

Press 0.4 with the numeric keypad, then press the enter button to complete the 0.4J/cm2 energy setting.

Press the start button to start irradiation, and the display screen will show the remaining energy. After irradiation is completed, the cross-linking instrument will automatically stop irradiation.

Remove the irradiated cells and turn off the power of the cross-linking device.

Purple diplomatic linkage instrument for membrane fixation:

In order to fix nucleotides on the membrane, the traditional method is to place the membrane in a vacuum oven at 80 degrees Celsius for 2 hours, which only takes 30-60 seconds on a UV Vis spectrophotometer. Compared with vacuum baking, the UV probe increases the hybridization signal intensity. The cross-linking instrument is equipped with an ultraviolet sensor inside, which can compensate for the output offset caused by the aging of the ultraviolet bulb.

Purple Diplomacy Analyzer for Polymer Curing:

Single cell immunoblotting technology (scWestern) uses an expandable open microporous array gel chip to drive cells to settle into the hole by gravity. After chemical lysis of cells and integration of SDS-PAGE electrophoresis to separate the proteins released by cell lysis, the photosensitive gel is activated by a short time of ultraviolet stimulation (~60s), and the gel is cross-linked with the protein to fix the protein in the gel. UV crosslinking has replaced the cumbersome membrane transfer process in traditional Western blot, achieving the analysis of expression levels of multiple proteins within a single cell within 4 hours. ScWestern combines the molecular sieve effect of SDS-PAGE, ensuring the specificity of detection through dual verification of protein molecular weight and antigen antibody recognition. It can detect cell surface proteins, transmembrane proteins, intracellular and nuclear proteins, and distinguish epigenetic modified proteins. Single cell immunoblotting technology can achieve high-throughput and multi index co detection, and study complex cell populations at the single-cell level. It has important application value in drug screening, cell heterogeneity research, and other fields.

The UCL-3500 ultraviolet radiation analyzer is a high-power, complete, microprocessor controlled ultraviolet irradiation system mainly used to connect nucleic acids to membranes and eliminate PCR contamination. The programmable microprocessor continuously monitors ultraviolet light emission. When the irradiated energy matches the pre-set energy, the irradiation will automatically stop. No matter how the intensity of the ultraviolet lamp fluctuates, the irradiation energy can be reproduced. The UCL-3500 ultraviolet radiation analyzer is equipped with an aluminum alloy UV irradiation chamber and an aluminum diffuse reflection plate, and the irradiation intensity inside the UV irradiation chamber is uniform. The ultraviolet sensor located above the irradiation chamber is installed with quartz glass to protect the sensor from chemical contamination.

Laboratory short wave ultraviolet cross-linking sterilization box

The UV Vis analyzer is used to eliminate amplicons during the preparation of PCR samples

The UV wavelength (nm) is generally selected as 254nm, and irradiation for 30 minutes is sufficient. It should be noted that when choosing UV to eliminate residual PCR product contamination, the length of the PCR product and the distribution of bases in the product sequence should be considered. UV irradiation is only effective for long fragments above 500bp and has little effect on short fragments. When exposed to UV radiation, pyrimidine bases in PCR products form dimers, which can cause elongation termination. However, not all pyrimidines in DNA strands can form dimers, and UV radiation can also cause dimer breakage. The degree of dimer formation depends on the UV wavelength, the type of pyrimidine dimer, and the sequence of nucleotides adjacent to the dimer site. On the irradiated long DNA strand, the number of dimer defects formed is less than 0.065/base, and other non dimer light damage (such as cyclobutane pyrimidine complexes, thymidine ethylene glycol, cross-linking between and within DNA strands, and DNA breakage) can terminate the extension of Taq DNA polymerase. The number of these sites is equivalent to that of dimeric sites. If these sites (0.13/base) are randomly distributed on a DNA molecule, and there are 32 damaged sites on a 500bp DNA molecular chain, then each of the 105 such molecules will have at least one damaged site. On the contrary, if there are only 6 damages on each chain of a 100bp fragment, many of the 105 copy molecules will have no damage. This is why UV irradiation has a certain segment length limitation.

Photocrosslinking for hydrogels:

The UCL-3500 series high-power ultraviolet radiation analyzer is a versatile ultraviolet radiation system mainly used to crosslink DNA or RNA onto nylon or nitrocellulose membranes. The cross-linking process only takes 25-50 seconds. The traditional method is to place the film in a vacuum oven and bake it at 80 ℃ for 2 hours. Compared with vacuum oven baking, irradiation with a UV probe can significantly increase the hybridization signal.

The UCL-3500 series high-power UV crosslinking analyzer can be used for membrane crosslinking in Northern, Southern blotting, EMSA, and other fields, CLIP-seq、iCLIP-seq、PAR-CLIP-seq、pBpa、sulfo-SDA、psoralen Such bimolecular cross-linking and microbial inactivation (such as bacteria, fungi, viruses), photostability verification, DNA cutting in agar gel, RecA mutation screening, partial restriction enzyme digestion produced by thymic dimer, UV sterilization to eliminate PCR pollution. It is also used in UV sterilization, polymer curing (such as hydrogel, methyl methacrylate resin), etc.

In order to achieve optimal cross-linking performance, each UCL-3500 series high-power UV crosslinking instrument is equipped with a UV radiation sensor (also known as a UV energy meter) for UV energy measurement, which is used to compensate for the aging of UV lamp power output. The equipment is calibrated according to standards before leaving the factory, ensuring the accuracy and consistency of radiation energy regardless of the strength and time difference of the ultraviolet light source.

Hydrogel is a kind of gel with water as the dispersion medium, which has a highly hydrated and swelling insoluble three-dimensional cross-linked network structure. Due to the diversity of its chemical structure and mechanical characteristics, hydrogels can highly fit the microenvironment of biological tissues, provide an effective carrier or scaffold material for the growth of cells or tissues, and have broad application prospects in the biomedical field. Especially in the field of tissue engineering and regenerative medicine, hydrogel products have many different functional applications. They can be used to fill defects, transfer bioactive molecules and cell tissues, transplant cells, regenerate blood vessels and organs, etc.

With the development of biomaterials science and tissue engineering, researchers have gradually realized the influence of the physical and chemical properties of the surrounding matrix on cell behavior. Environmental changes such as matrix hardness, surface morphology, and growth factors around cells can affect physiological activities such as cell growth, metabolism, differentiation, and migration. Therefore, the artificial construction of biomimetic 3D extracellular matrix using hydrogels is of great biological and medical significance, especially for the basic research of cell biology, such as the induction and differentiation of stem cells, the signal transduction of nerve cells, the construction of organ models for drug screening, and the use of hydrogels as cell carriers in tissue engineering and regenerative medicine and other applications.

In recent years, in order to realize the construction of hydrogel microenvironment, light as an effective control method has been applied to the construction of hydrogels and the regulation of microenvironment properties. Compared with other control methods, light regulation has unique advantages, including spatiotemporal controllability, non direct invasion, and low biological toxicity. In summary, based on the influence of cellular microenvironment on cell fate, the physical and chemical properties of materials can be controlled in time and space through light regulation technology, enabling the cultivation of cells on the surface or inside biological materials, and real-time observation and guidance of cellular physiological processes. This has become a new trend in modern biological research. This paper briefly introduces the classification, preparation and application of hydrogels, focusing on the construction of hydrogels through photochemical reaction, the regulation of physical and chemical properties of hydrogels, and their applications in the field of biomedicine.

The UCL-3500 series high-power ultraviolet radiation analyzer is equipped with six 15W high-power ultraviolet lamps, which have high irradiation intensity and large irradiation area, and can quickly irradiate multiple sets of experimental materials. Spot supply of short wave (UVC254nm), medium wave (UVB 302nm), and long wave (UVA 365nm) ultraviolet diplomatic coupling devices.