LabChip GX Touch Nucleic Acid Analyzer

The LabChip GX Touch system has the following functions:

²LabChip microfluidic technology for modern gel electrophoresis analysis

²Fully automated real-time analysis of genome samples, sample detection and analysis can be performed as quickly as possible30 seconds

²Digital data format for easy analysis, viewing, sharing, and archiving

²Quantitative indicators of RNA and DNA sample integrity to ensure that only the best samples can enter downstream processes

²Highest batch processing throughput Efficient platform for 24 samples saves time and reagent usage;

Highest batch processing throughput The 384 sample models are suitable for high-throughput workflows

Whether your sequencing laboratory needs to analyze scarce and precious samples, or run The PCR free process, or the need to quantify each fragment in the sample for genotyping analysis, makes the LabChip GX Touch nucleic acid analyzer convenient, low-cost, high-throughput, and flexible, which can greatly reduce the burden of analyzing an increasing number of samples.

The LabChip GX Touch touchscreen simplifies sample analysis:

1. Touch Humanized Operation

²Load sample board and chip

²Select sample (maximum run at once) 384 samples)

²Select experimental type

²contact point'Run', start the experiment

²You can even have the system automatically export the data directly to your website

²Network or LIMS system (Figure 2)

picture2. Data analysis starts with an easy-to-use touchscreen interface

2. run Real time observation of operation

²Sample analysis takes as little time as 30 seconds

²Real time visualization of electrophoresis peaks during data collection process

²Overlay the collected data in the operating environment and compare the sample spectra

²Select different runtime analysis feature annotations

3. Review Real time viewing of data or exporting for future analysis

²Choose to display in the form of electrophoresis peak map, virtual gel map, or data table (Figure) 3）

²use TIBCO Spotfire ® Data visualization software enhances analysis results

²Collect data from multiple board samples for parallel analysis and comparison

²Select and filter data with key attribute applications

²Highlight the target peak spectrum

picture3. Flexible data options allow real-time viewing or exporting in multiple formats

The LabChip GX Touch software can conduct experiments with minimal settings. The experimental setting parameter file can be downloaded to the instrument and executed through touch buttons. Automatically retain the experimental parameters of the most recent experiment run and continue running multiple batches of samples.

The software module can help scientists view completed samples from work far away from the laboratory, making it easier to view the same data. The software's usage license has unlimited restrictions, and colleagues can share data, making project collaboration simple and direct.

LabChip GX Touch data files are compatible with TIBCO Spotfire (optional feature).

Only send high-quality nucleic acid samples downstream

Electrophoretic analysis is an important step in the quality control of second-generation sequencing libraries, as it can simultaneously provide fragment size and quantitative information for each sample.LabChip GX Touch automatically completes fragment size and concentration analysis of DNA and RNA fragments, as well as smear samples, for quality control of library samples used in sequencing. Fragment sample analysis is commonly used for cross checking the effectiveness of fragmentation or PCR steps. The DNA total analysis of library samples was performed using homogenization steps.

Save samples

Reducing sample consumption during analysis is an important factor considered by many laboratories. For tasks such as chromosome immunoprecipitation sequencing and It is crucial to prepare library samples with very low concentrations using PCR free and other methods. The NGS 3K kit is specifically developed for sequencing processes that require particularly high sensitivity. LabChip can handle concentrations as low as 2.5pg/μ L

of Accurate quantification of smear and 0.2 pg/μ L fragments enables rapid analysis of scarce and precious samples (Figure 4).

pictureThe above figure shows the sensitivity of DNA NGS 3K detection samples. The same NGS library sample was diluted 6 times in a 3-fold series, and the experimental results showed the lower limit of LabChipDNA NGS 3K detection sample concentration. The data results are displayed on the LabChip GXTouch instrument.

quick analysis RNA

The LabChip Touch platform provides easy to understand RNA concentrations and ratios of ribosomal RNA, which are used together with RNA quality scores as integrity evaluation indicators (Figure 5). RNA Quality Score (RQS) is a quantitative evaluation of the quality and integrity of a sample, which can predict the success rate of downstream gene expression experiments such as chip analysis or real-time PCR.

except In addition to RQS values, the analysis software can now automatically calculate DV200 values. These two values can be exported and used to determine the quality of RNA samples from specimens such as FFPE. Verified, RQS and Agilent^® Also （ RNA

The integrity index is closely related and follows the same criteria Quantitative rating from 0 to 10.

genome DNA sample degradation score

for The genome DNA experiment developed by LabChip GX Touch system innovatively introduces the genome DNA quality score (GQS), replacing the traditional gel electrophoresis. The electronic data provided is compatible with laboratory information management systems and can be easily archived and distributed. Can quickly analyze DNA samples of 25-12000bp within 30-60 seconds (with an upper limit of 40000 bp for gDNA integrity detection) (Figure 6).

picture5. RNAElectrophoretic map, displaying18Sand28Speak

picture6. Genome displayed by electrophoretic mapDNA quality assessment

Small RNA analysis

The LabChip small RNA kit is capable of rapidly isolating and determining various small RNA samples with fragment ranges between 20 and 150 nucleotides (nt), including miRNA, siRNA, piRNA, tRNA, and CRISPR gRNA. This new small RNA analysis method can quickly and automatically analyze the size and relative abundance of small RNA fragments, thereby achieving time and cost savings.

LabChip microfluidic capillary electrophoresis technology can automatically separate and analyze small RNA samples within one minute. The system software automatically analyzes the data and determines the fragment size and concentration of small RNA samples by using internal reference and standard samples (Figure 7).

picture7.LabChipsmallRNAThe detection method can automatically measure smallRNAThe fragment size of the sample, Has high accuracy and precision.

Labchip electrophoresis

working principle

LabChipElectrophoresis is performed on a small microfluidic chip (Figure)8）. Loading the chipLabChip GX TouchAfter the system, the chip hole is in contact with the platinum electrode, which provides voltage and current control. The system robotic arm moves the sample well on the microplate directly below the capillary "pipette" of the chip, approximately150 nLThe sample is drawn onto the chip. The samples to be analyzed are separated by electrophoresis one by one, and the sample bands are detected by laser-induced fluorescence. throughladderDetermine the size and concentration of each band using internal standards.

picture 8.Working principle of Labchip electrophoresis

LabChip GX Touch Reagent specifications

Order Information