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Jeko-1+LUC human mantle cell lymphoma cells+LUC
Cell Introduction
name |
JeKo-1 (Human mantle cell lymphoma cells) (STRCorrectly identified) |
alias |
Jeko-1; JEKO-1; JeKo 1; Jeko1; JEKO1; JEKO |
genus |
human |
Age (gender) |
woman78year |
source of organization |
Organ: peripheral blood; Organization: mantle cell lymphoma |
Growth characteristics |
suspended cells |
cell morphology |
Lymphocyte like |
Background description |
A giant cell variant of a mantle cell lymphoma patient showed leukemia transformation, established from peripheral blood mononuclear cellsMCLcell lineJeKo-1.JeKo-1cellEBVirus negative and expressing aBThe phenotype of cellsIgM.JeKo-1Cell overexpressioncyclin D1TheBcl-2Thec-MycandRbProtein.Bcl-1/J(H)Gene rearrangement has been obtainedPCRconfirm,JeKo-1Cells inSCIDHigh tumorigenicity in mice. |
Biosafety level |
1 |
Growth medium |
RPMI-1640+20% FBS+1% P/S |
Recommended passage ratio |
3×10^5-2×10^6cells/mL |
Recommended fluid change frequency |
2~3time/week |
Doubling time |
~26-50hour |
Freezing conditions |
Freezing solution:55%Basic medium+40%FBS+5%DMSOTemperature: Liquid Nitrogen |
culture conditions |
Gas phase: air,95%;CO2,5%temperature37℃ |
Antigen expression status |
CD3-, CD5+, CD10+, CD19+ (verified at ATCC) |
Gene expression status |
CD3-, CD5+, CD10+, CD20+ (verified at ATCC) |
Precautions |
This cell is a suspended cell, please collect the cell suspension by centrifugation; Do not directly discard the cell culture medium. |
Preservation institution |
ATCC; CRL-3006 DSMZ; ACC-553 |
Jeko-1+LUC human mantle cell lymphoma cells+LUC
