Bovine pituitary extract (BPE)

Product concentration: 14mg/ml concentration
Extract of bovine pituitary gland(Bovine Pituitary Extract，BPE)It is a mixture extracted from bovine pituitary gland, containing various bioactive substances such as proteins, hormones, and peptides.Has the following properties:

1Biological activity:BPEContaining various bioactive substances such as growth factors, hormones, and cytokines, it has a promoting effect on cell growth, proliferation, and differentiation;
2Promote cell culture:BPECan provide necessary growth factors and nutrients during in vitro cell culture to promote cell proliferation and growth;
3Protein source:BPERich in protein, it can be used as a protein source in culture media, providing the amino acids and nutrients needed by cells;
4Solution form:BPEIt usually exists in the form of a solution, which is convenient for use in cell culture.

Function:

BPEIts main use is in the field of cell culture, especially in low serum or serum-free conditions, it is widely used as a promoter to increase cell growth and activity, which can accelerate cell proliferation, enhance cell growth and differentiation ability. can be paired withMCDB131Series orDMEM/F-12Combination use of basic culture media.

productConcentration:14mg/mL

Dilution ratio:1:300-1:1000Either way, the usual dilution ratio is1:400

store/保存方法：-20℃ storage

common problem:

1Is the bovine pituitary extract solution sterile?

Answer: It is a sterile solution.

2Is the presence of flocculent material after thawing a normal phenomenon?

Answer: It is normal to see flocculent material before opening and using it after thawing, which is some brain tissue fragments and does not affect the experimental effect. However, strict aseptic measures must be taken during the overall operation process.

3Does this flocculent substance need to be removed?

Answer: It can be removed or not removed. If you want to remove it, you can300g，4℃, centrifugation5minTake the supernatant and use it.

4GenerallyBPEWhat is the recommended amount to be added to the culture medium?

Answer: The recommended range varies depending on the type of cultured cells and their intended use25-70ug/mLA typical addition concentration value is50µg/mL. Confirm * BestBPEAn effective method to add concentration is to perform growth assays on selected cells.