Irrigation bioreactor

BioTenseIrrigation bioreactorA perfusion bioreactor used for studying mechanical signal transduction in mechanobiology

As the next generation instrument,BioTenseThe design of perfusion bioreactors aims to assist tissue engineers and cell biologists in high-resolution research in mechanobiology.BioTenseThe advantage lies in its ability to apply uniaxial mechanical stimulation and allow for long-term direct observation of cells at high magnification/Matrix interactions.BioTenseIt is an independent instrument that can apply7NThe uniaxial force and25mmThe elongation rate.BioTenseLocated in the inverted microscopeXYZOn the loading platform..

So far, the instrument has successfully recordeda)The process of proliferation, growth, convergence, and stratification of fibroblasts on different matrices;b)Complex and organized cell membrane movement;c)Coordinate the production of extracellular matrix. Like all of our testing systems,ADMETCustomize itBioTenseDesign to meet your needs.

Mechanical biology focuses on the ways in which cells perceive and respond to mechanical stimuli. A major challenge in this field is understanding mechanical signal transduction. Mechanical signal transduction refers to the process by which the body converts mechanical loads into cellular responses.BioTenseA bioreactor is a groundbreaking instrument specifically designed for studying mechanical signal transduction.

BioTenseThere is an adjustable height chamber to minimize dead space to the greatest extent possible（< 2mLPossible), thereby reducing the interaction with perfusion media and exogenous stimuli/The costs associated with inhibitors, proteases, and labels.

Main features:

Can promote long-term proliferation and differentiation of cells

Maintain the required nutrient concentration through infusion process

Expose the organization to uniaxial force and strain fields

Provide a window for direct observation of cell activity under a high magnification microscope

Accurately control the temperature of the reactor

Reduce dead chamber volume to a maximum extent through adjustable height chambers

(A)Initial planting(B)Proliferation(C)The initial response to strain and(D)Time series of final morphological response of primary human corneal fibroblasts on dense collagen matrix (superimposed)DIC/Fluorescence imaging.BiotenseThe combination of functions in the system allows for real-time, high magnification imaging of the response of the cell culture system to long-term (several weeks) applied loads or strains.

Image from Northeastern UniversityR. ZareianandJ. W. RubertiFriendship provided

feature

Main chamber: composed of chamber cover, chamber observation port, and bioreactor frame. The chamber cover includes heating elements, thermocouples, and a window for observing the sample. The chamber viewport can be vertically adjusted and allows the objective lens to approach the sample, placing it at a high numerical aperture60xWithin the working distance of the objective lens. A framework is a structural component that supports and positions all major components. It isolates the bioreactor from the microscope and is used to position the bioreactor on the microscope stage. All components in contact with the fluid in the chamber are biocompatible and can be sterilized between uses.

Optical access:BioTenseThe main feature is its adjustable viewport, which can be used up to600High magnification optical microscopes achieve continuous and long-term imaging.

Chamber volume:In order to minimize blind spots to the greatest extent possible, the design of adjustable viewports and fixtures ensures that the sample is located at objects in the lower part of the chamber. By wisely selecting the sample size, the chamber volume can be as small as2mL.