Ion exchange chromatography column Proteomix 403NP5-4605

Competition scoreProteomixThe ion exchange column is specially designed for the separation of proteins, oligonucleotides, and peptides, and has the characteristics of high resolution, high column efficiency, and high recovery rate. The filler matrix is rigid, spherical, and highly cross-linked polystyrene-Diethylbenzene(PS/DVB)particle Ionic exchange columns are divided into porous and non porous typesPS/DVBTwo. porousPS/DVBThe size of resin particles varies5MmThe10MmThe aperture is500 Oh.The size of resin particles without pores is1.7MmThe3MmThe5MmThe10Mm.PS/DVBThe resin surface is bonded with a highly hydrophilic neutral polymer thin layer of nanoscale thickness. hydrophobicPS/DVBThe surface of the resin is completely covered with this hydrophilic material, thereby eliminatingPS/DVBThe irreversible adsorption of biomolecules ensures high separation efficiency and biological sample recovery rate. By using the unique chemical bonding technology of Saifen, ion exchange functional groups can be densely and uniformly bonded to the surface of the hydrophilic layer.

Stability and performance

Due toPS/DVBThe surface modification is entirely based on chemical bonding, soProteomixIon exchange columns have excellent stability. This stationary phase can interact with most buffer solutions such as ammonium acetate, phosphateTrisWaiting for compatibility. inpH 6.0At that time20 mMPhosphate buffer solution operates as the mobile phaseProteomix SCXandWCXChromatography column, or inpH 8.0At that time20 mM TrisBuffer salt operates as the mobile phaseProteomix SAXandWAXchromatographic column1000 Secondary or usage3 Within a month, the column effect remained almost unchanged.

picture2.useProteomix SCX-NP3Atlas obtained by separating protein mixtures

Proteomix SCX-NPTheWCX-NPTheSAX-NPTheWAX-NPResin with no poresPS/DVBThe particles serve as the matrix, with a hydrophilic layer covering the surface and a uniform layer of ion exchange functional groups bonded together. These stationary phases have three characteristics. Firstly, the hydrophilic layer with nanoscale thickness completely eliminates non-specific interactions between the carrier and the biological sample. Secondly, the pore free particle structure minimizes the lateral diffusion of the sample while suppressing its diffusion into the interior of the filler particles. Thirdly, Saifen's special GD technology can synthesize a uniform and dense ion exchange layer. Specially designed by DProteomix SCX-NPTheWCX-NPTheSAX-NPandWAX-NPThe stationary phase can provide the best resolution and separation efficiency for proteins, oligonucleotides, carbohydrates, and peptides. picture2 isProteomix SCX-NPpillar(4.6x50 mm，3 Mm)Regarding three proteins (ribonucleases)ACytochromeCThe separation results of lysozyme. Lysozyme in5cmThe separation efficiency on the long column has reached100,000Theoretical tray, this separation effect is unprecedented before D.

picture3 to useProteomix SAX-NP5Column separation of ovalbumin andBSAA chromatogram obtained from the mixture.Proteomix SAX-NP5The extremely high resolution and selectivity not only enableBSADimer andBSASeparate, and impurities in the egg white protein mixture have also been separated.

Proteomix SCX-PORTheWCX-PORTheSAX-PORandWAX-PORThe filler is porousPS/DVBThe particles serve as the matrix, with a hydrophilic layer covering the surface and a uniform layer of ion exchange functional groups bonded together. The aperture is500 Oh.This stationary phase has three characteristics. Firstly, the nanoscale thick hydrophilic layer completely eliminates non-specific interactions between the carrier and the biological sample. Secondly, Saifen's special D technology can synthesize uniformly dense ion exchange layers. Thirdly, porous particles have a large specific surface area, resulting in high separation capacity.Proteomix SCX-PORTheWCX-PORTheSAX-PORandWAX-PORIt has extremely high separation efficiency and separation capacity for proteins, oligonucleotides, peptides, etc. This porous filler is particularly suitable for semi preparation, separation, and purification of biomolecules.

Proteomix SCXTheWCXTheSAXandWAXThe inner diameter of the chromatographic column is0.75、2.1、3.0、4.6、7.8、10and21.2 mmThe length is2、3、5、10、15、25and30 cmSai Branch can also customize chromatography columns according to customer requirements.