In vitro model simulation reactor for gut microbiota

In vitro model simulation reactor for gut microbiota

The important bottleneck in the study of gut microbiota lies in the collection of samples. For normal people, besides collecting feces, sampling from the small intestine, ascending colon, transverse colon and other parts is almost impractical. On the other hand, due to the dual influence of external environment and host intestinal environment on gut bacteria, it is crucial to distinguish the roles of external environment and intestinal environment on gut microbiota. Therefore, establishing a reasonable and easy to operate in vitro model is of great significance for promoting the development of intestinal microecology, human and animal nutrition.
In vitro model simulation reactor for gut microbiotaWidely applicable to gut microbiota laboratories and nutrition laboratories in universities, research institutes, and enterprises, SHIME is an ideal tool for in vitro gut model experiments, including batch culture models, continuous fermentation culture systems (ascending colon, transverse colon, and descending colon), and human gut microbiota simulators (stomach, small intestine, ascending colon, transverse colon, descending colon). Can be applied to the study of bacterial function and diversity within the gut microbiota, in vitro simulation of the pharmacological effects of gut antibiotics on gut microbiota changes, and research on the production of microbiota preparations and probiotics; The extracorporeal digestion simulation device can simulate the real intestinal environment, optimize and screen process parameters, and monitor and control various parameters of intestinal dynamics online.

Technical Specifications

1. Control the constant temperature of the gastrointestinal tract; Temperature can be controlled between 30-40 ℃; Accuracy: ± 0.1 ℃; Intelligent PID temperature control; Adopting a brand new semiconductor waterless temperature control system;

2. Control the physical and chemical environment in the gastrointestinal tract; Peristalsis, acidity, slight positive pressure, anaerobic environment;

3. Automatically detect and control the acidity of different simulated parts;

4. Perform enzymatic hydrolysis reaction and simulate the secretion of digestive enzymes in the gastrointestinal tract;

5. Detecting the chemical changes in "food" during the digestion process;

6. Simulate small intestine endocrine digestive enzymes to further digest and degrade substrates; At the same time, provide an environment for the growth of various microorganisms in the gastrointestinal tract of monogastric animals;

7. Simulate multiple absorption methods and perform active and passive absorption based on the absorption characteristics of different parts;

8. The secretion volume and rate of digestive and secretion fluids can be regulated within the range of 0-150ml/min; Parameters can be set and modified by oneself;

9. The experimental repetition rate deviation is less than 1%; Absorption deviation<2%;

10. Easy to operate and assemble, the program can automatically analyze data and draw curves, and the system comes with multiple simulated process formulas;

11. The server adopts WFC remote communication mode;

12. The client development adopts Siemens programmable on-site control and dual monitoring and control on the upper computer end;

13. The device adopts dual screen data synchronization display, which is intuitive and convenient;

14. Cloud based development system, which can be operated through an APP on mobile devices, as well as cloud data storage; Easy to use.