In situ rhizosphere enzyme activity imaging system

LK-1760In situ imaging of rhizosphere enzyme activitysystem

LK-1760In situ imaging of rhizosphere enzyme activitysystemBased on the principle of fluorescence quenching generated by the reaction between fluorescently labeled substrates and soil enzymes,Being able to visualize and analyze enzyme activity in rhizosphere soil without damaging the soil and roots, in order to gain a deeper understanding of soil-root system-The interactions between microorganisms and the functions of soil enzymes in ecosystems provide powerful tools.

Main Features

lin situ detection：Directly detecting enzyme activity in soil and root environment to avoid sampling errors

lvisual analysis：Intuitively display enzyme activity distribution through fluorescent signals, easy to understand and analyze

lhigh sensitivity：Can detect low enzyme activity and capture subtle changes

lnon-destructive：Protect soil and root integrity, reduce ecological damage

lIntelligent image processing software:It is possible to obtain the average enzyme activity and enzyme activity intensity distribution of soil profiles, visualize the enzyme activity distribution, and quantify the relationship between enzyme activity and root distance.

lwidely used：Not only can it be used to study the influence of plant roots on soil enzyme activity, but it can also be used in multiple fields such as monitoring environmental pollution, soil improvement, crop breeding, etc

Technical Specifications

lMeasurable types:β -Glucosidase, cellulase, xylanase, chitinase, phosphatasewait

lbecomeLike a carrier:Polyamide filter membrane

lFilter membrane pore size:0.45um

lFilter membrane size:200mm*200mm(Customizable)

lFluorescent labeled substrate

lHatching time:≤60minute

lImaging conditions

Excitation light wavelength:355nmultraviolet

Emission wavelength:460nmblue light

lImaging system:1/1.8'CMOSGigabit Ethernet industrial area array camera;

Maximum frame rate:5.9fps；

Dynamic range:61dB

Signal to Noise Ratio:39dB

Image resolution:2000Ten thousand pixels

lRepetitive error:less than or equal to5%；

lGrayscale value analysis:16 bit

lMain accessories:Excitation light sourceLEDmatrix1Set; Ethernet industrial array camera1One;450nmoptical filter1One; Incubation filter membrane100Zhang; Shooting in the dark box1One; Substrate reagent1Set; Image analysis software1Set.

lImage processing software:It is possible to obtain the average enzyme activity and enzyme activity intensity distribution of soil profiles, visualize the enzyme activity distribution, and quantify the relationship between enzyme activity and root distance.

Application effect