IL6R KO STAT3-Luc HEK293

NegotiableUpdate on 05/06

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

IL6R TO STAT3-Luc HEK293

Product Details

IL6R KO STAT3-Luc HEK293

I. Background
The characteristic of cancer-related inflammation is the presence of specific inflammatory cells and mediators, including cytokines and chemokines. Recent evidence suggests that the signal transduction and transcription activator (STAT) family proteins, particularly STAT3, play a crucial role in selectively inducing and maintaining the pre cancerous inflammatory microenvironment during the onset of malignant transformation and cancer progression. Signal transduction and transcription activator 3 (STAT3) is a transcription activator that directly transmits signals from extracellular receptors to the nucleus, mediates the expression of some genes, and plays an important role in cellular processes such as apoptosis. Under the action of cytokines and growth factors, STAT3 is phosphorylated by receptor associated Janus kinase (JAK), forming homodimers or heterodimers, and translocated into the nucleus to act as a transcriptional activator. STAT3 signaling is the main intrinsic pathway of malignant tumors, as it is frequently activated in malignant cells and plays a critical role in regulating many genes crucial for cancer inflammation in the tumor microenvironment.

II. Description
IL6R KO STAT3-Luc HEK293Report gene cells and knock out IL6R on STAT3 Luc HEK293 cells. Ligands andIL6R KO STAT3-Luc HEK293Combining and activating the JAK/STAT3 signaling pathway, the principle is shown in the following figure. Figure 1.IL6R KO STAT3-Luc HEK293Schematic diagram of cell model

III. Introduction
Knockout genes:	IL6R
Expressed gene:	IL6R KO STAT3-Luc
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	DMEM+10%FBS+100 μg/ml Hygromycin B
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen

IV. Description of Host Cell Line
Organism:	Homo sapiens, human
Tissue:	Embryonic kidney
Disease:	Normal
Morphology:	Epithelial
Growth Properties:	Adherent

V. Representative Data
Figure 2. Sanger ofIL6R KO STAT3-Luc HEK293. Figure 3. Dose Response of Samples inIL6R KO STAT3-Luc HEK293(C15). Figure 4. Dose Response of Samples inIL6R KO STAT3-Luc HEK293(C15).