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Bixin Biotechnology (Hainan Province) Co., Ltd
IL-6 detection kit (flow cytometry)
NegotiableUpdate on 05/06
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Overview
IL-6 detection kit (flow cytometry). $r $nIL-6 can regulate the growth and differentiation of various cells, regulate immune response, acute phase response, and hematopoietic function, and play an important role in the body's anti infection immune response. There are many target cells that L-6 acts on, including macrophages, liver cells, quiescent T cells, activated B cells, and plasma cells
Product Details
IL-6 detection kit (flow cytometry)Using flow cytometry (CBA)，Cytometric Bead Array）Detecting Cytokine IL- 6，
CBA, also known as Micro Sample Multi Index Flow Cytometry, is a multi protein quantification detection method based on flow cytometry detection system, which can simultaneously detect multiple indicators in a single sample. The testing service of Bixin Biotechnology is based onCBAPrinciple: Connect corresponding cytokine antibodies to specific fluorescent encoded microspheres. When specific antigens are detected in the sample, these capture antibodies linked to fluorescent encoded microspheres will bind to them, and finally, phycoerythrin will be added（PE）Labeling specific detection antibodies to form microsphere linked capture antibodies-antigen-PEThe labeled detection antibody complex. Phycocyanin in flow cytometry（APC）And phycocyanin-Cy7（APC-Cy7）Under the channel, microspheres are recognized and used to determine antigens; In flow cytometryPEUnder the channel, on the microspherePEThe fluorescence intensity is positively correlated with the concentration of each detected substance in the sample, and the corresponding fluorescence signals of each calibration standard can be fitted into a concentrationThe fluorescence intensity standard curve can be used to calculate the concentration of each antigen through the curve equation.
Testing process:
Advantages and Characteristics
1. Able to perform multiple tests on a single sample simultaneously.
2. The required sample size is small, with serum (5-10 μ L) and cell supernatant (25-50 μ L), saving sample size compared to traditional detection.
3. High sensitivity and good repeatability.
4. All analyses only require one set of standard curves.
5. Avoid artificial illusions caused by enzyme-linked reactions.
6. Using magnetic fluorescence encoded microspheres for convenient operation and saving detection time
Optional detection indicators: (This product is IL-6 detection kit (flow cytometry), TestingIL- 6Alternatively, other cytokines can also be selected for combined detection
小鼠细胞因子: IL-2、IL-4、IL-6、IL-10、IL-17A、IL-12p70、IL-1β、MCP-1、IFN-γ、TNF-α）
Human polycytokines: IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, TNF-α, IFN-γ, IFN-α2)
Bixin Biotechnology (Hainan Province) Co., Ltd. is a high-tech biotechnology enterprise specializing in the independent research and development of magnetic fluorescence encoded microspheres and the development of flow cytometry detection multi protein assay kits. The company is composed of a doctoral team with solid research foundations and rich experimental experience in multiple disciplines such as biology, materials, and medicine. Three sizes of low adsorption magnetic fluorescence encoded microspheres with approximately 30-70 fold fluorescence have been developed, and a flexible multi cytokine detection kit has been further developed based on this.
