- Phone
-
Address
Xigang District, Dalian City, Liaoning Province
Product Categories
Mulikang (Dalian) Medical Technology Co., Ltd
IBa1 antibody, rabbit source (for immunohistochemistry) iba1
NegotiableUpdate on 05/07
- Model
- Nature of the Manufacturer
- Producers
- Product Category
- Place of Origin
Overview
Iba1 (Ionized calcium binding adapter molecule 1) is a calcium binding protein with a molecular weight of approximately 17 kDa. Due to its specific expression in microglia in the central nervous system, Iba1 is used as a marker for microglia.
Product Details
Iba1 antibody, rabbit source (for immunohistochemistry)
Anti Iba1, Rabbit (for Immunocytochemistry)
Iba1 (Ionized calcium binding adapter molecule 1) is a calcium binding protein with a molecular weight of approximately 17 kDa. Due to the specific expression of Iba1 in microglia in the central nervous system1）Therefore, it is used as a marker for microglia cells. According to reports, Iba1 is expressed in both resting and activated microglia, but there are reports that its expression increases in activated microglia2）In addition, Iba1 is also expressed in macrophages of peripheral tissues, known as AIF-1 (Allograft Inflammatory Factor 1, Allograft inflammatory factor-1）。 Iba1 binds to F-actin inside cells and plays a role in forming actin fiber bundles. The formation of this actin fiber bundle is considered necessary for observing the formation of cell membrane wave structures during cell migration and phagocytosis processes3）.
Anti Iba1, derived from rabbits (for immunohistochemistry), can be used to stain microglia, including processes, in immunohistochemical staining. Therefore, it has been used by researchers around the world as a standard for labeling antibodies against microglia.
In recent years, microglia have attracted much attention due to their neurotrophic and protective effects, as well as their neuroprotective effects caused by the production of NO, TNF - α, and IL-1 β.
Stained image of microglia
Anti Iba1， Rabbit source (for immunohistochemistry)
◆ Characteristics
In immunohistochemical staining (frozen sections), microglia including their processes can be stained※
The concentration used is 1:500-1:1000, and it can also be used in small amounts for staining.
The degree of staining depends on the condition of the sample and cannot guarantee successful staining.
◆ Antibody information
Types of antibodies |
polyclonal antibody |
antigen |
The synthetic peptide of Iba1 (with the same C-terminal sequence) |
Immune animals |
rabbit |
intersectionality |
Mice, rats, and others※ |
concentration |
0.5-0.7 mg/mL |
application |
Immunohistochemical staining (frozen section): 1:500-1000 Immune cell staining: 1:500-1000 |
Storage conditions |
-20 ℃ frozen storage (dry ice transportation) |
Antigen derived animals |
rat |
※ There are others4）、dog5）The cat6）Pigs7）The marmoset monkey8）Zebrafish9）Application examples
clickhereView usage papers
◆ Application data
Immunohistochemical staining (mouse)
|
Species: Mouse Location: cerebellum Sample: Frozen section Antibody concentration: 1:1000 |
|
Species: Mouse Location: nucleus accumbens Sample: Ultra thin slice Antibody concentration: 1:500 <Data Provision> Professor Hagiwara and Mr. Yamazaki from the Department of Life and Biological Sciences at Tokyo University of Science |
|
|
Species: Mouse Location: Spinal cord Sample: Frozen section Antibody concentration: 1:500 <Data Provision> Professor Hagiwara and Mr. Yamazaki from the Department of Life and Biological Sciences at Tokyo University of Science |
|
Species: Mouse Location: Retina Antibody concentration: 1:1000 <Data Provision> Prof. Lieve Moons, KU Leuven, Belgium. |
Immunohistochemical staining (rat)
|
Species: Rat Location: cerebral cortex Sample: Frozen section Antibody concentration: 1:1000 <Data Provision> National Center for Psychiatry and Neuromedicine Research in Japan Teacher Sayaka, Teacher Makoto, Teacher Ichigo, Teacher Takasaka |
◆Anti Iba1 antibody series
Product Details |
Product grade |
Microglia marker antibody Anti Iba1 antibody |
Immunochemical use |
Anti Iba1, recombinant rabbit monoclonal antibody (6A4) |
Immunochemical use |
Anti Iba1, Rabbit (for Immunocytochemistry) |
Immunochemical use |
Anti Iba1, rabbit (for paraffin sectioning) |
Immunochemical use |
Anti Iba1, rabbit biotin binding |
Immunochemical use |
Anti Iba1, rabbit source (combined with 488 green fluorescence) (Prototype) |
Immunochemical use |
Anti Iba1, rabbit source, combined with SPICA Dye ™ five hundred and sixty-eight |
Immunochemical use |
Anti Iba1, rabbit source, SPICA Dye ™ 594 coupling |
Immunochemical use |
Anti-Iba1、 Red fluorescent dye (635) binding |
Immunochemical use |
Anti Iba1, goat polyclonal antibody |
Immunochemical use |
Mouse derived Iba1 antibody, unlabeled, monoclonal antibody (NCNP24) |
Immunochemical use |
Anti Iba1, rabbit derived monoclonal antibody (NCNP27) |
Immunochemical use |
※ For ophthalmic samples, immunohistochemistry (frozen section) is recommended to use:Anti Iba1, recombinant rabbit monoclonal antibody (6A4)
Product List
| Product Number | Product Name | Product Specifications | product grade | Remark |
| 019-19741 | Anti Iba1, Rabbit (for Immunocytochemistry) Anti Iba1, rabbit (for immunohistochemistry) |
50 μg | Immunochemical use | - |
Similar Product Recommend