Human triiodothyronine reagent kit, used for detecting serum and plasma T3 levels. ELISA or chemiluminescence methods are commonly used, which are sensitive, specific, easy to operate, and can assist in the diagnosis of thyroid diseases with reliable results.
Human triiodothyronine reagent kit
Human triiodothyronine reagent kitIt is a tool used for quantitative detection of triiodothyronine (T3) levels in human serum or plasma, and plays an important role in thyroid function assessment and related disease diagnosis. Here is a specific introduction:
Detection PrincipleCommon methods include enzyme-linked immunosorbent assay (ELISA) and chemiluminescence assay. For ELISA kits, the double antibody sandwich method or competitive binding principle is used. In the double antibody sandwich method, the anti-T3 antibody is first coated on a microplate, and after adding the sample, the T3 in the sample binds to the solid-phase antibody. Then, the enzyme-linked antibody is added to form an antibody antigen enzyme-linked antibody complex. The substrate is added for color development, and the color depth is proportional to the T3 content. The principle of competitive binding is that T3 in the sample competes with enzyme labeled T3 for limited antibody binding sites, and the color intensity is inversely proportional to the concentration of T3 in the sample4.
Product FeaturesIt has high sensitivity and can detect trace changes in T3 in serum, which is helpful for early detection of thyroid dysfunction; High specificity, the antibodies in the kit have been carefully screened and optimized to specifically recognize T3, avoiding cross reactivity with other thyroid hormones; Easy to operate, complete kit components, simple and clear operating steps, no need for complex instruments and equipment; The results are reliable, and the accuracy and reliability of the test results are ensured through a strict quality control system and standardized operating procedures4.
Scope of applicationMainly used to assist in the diagnosis of thyroid related diseases, such as hyperthyroidism, T3 elevation often precedes the decrease of thyroid stimulating hormone (TSH) and is one of the sensitive indicators for diagnosing hyperthyroidism; It can also be used for the diagnosis and monitoring of diseases such as hypothyroidism, T3 hyperthyroidism, iodine deficient goiter, and hyperthyroidism4.
operating procedureFirstly, collect venous blood samples and separate serum or plasma to avoid hemolysis and contamination. Then follow the instructions of the reagent kit for operation, which generally includes steps such as reagent preparation, sample addition, incubation, washing, and detection. If using an ELISA kit, incubate at a specific temperature after adding the sample to allow the antigen and antibody to fully react, wash to remove unbound substances, add the substrate for color development, read the absorbance value at the specified wavelength using an enzyme-linked immunosorbent assay (ELISA) reader, and then calculate the concentration of T3 in the sample based on the standard curve4.
