Human intrahepatic cholangiocarcinoma cell line ETK-1 (STR identification correct)

NegotiableUpdate on 01/28

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Overview

Product Code: JY-J1161 $r $n Product Name: Human intrahepatic cholangiocarcinoma cell line ETK-1 (STR identification correct) $r $n Product Specification: 1 #215; 10 ⁶ cells/T25 culture bottle $r $n Product brand: Jinyuan Biotechnology

Product Details

1、 Product specifications

Product Code: JY-J1161

Product Name:Human intrahepatic cholangiocarcinoma cell line ETK-1 (STR identification correct)

Product specifications: 1 × 10 ⁶ cells/T25 culture bottle

Product brand: Jinyuan Biotechnology

2、 Product Introduction

Human intrahepatic cholangiocarcinoma cell line ETK-1 (STR identification correct)

genus	person
alias	ETK1
Source of organization	intrahepatic bile duct
disease	cholangiocarcinoma
Passage ratio/cell digestion	1: 2 passages, digest for 2-3 minutes.
Wanquan culture medium configuration	RPMI1640 medium; 10% fetal bovine serum; 1% dual antibody
Introduction	The cells were derived from a liver metastatic, ascites derived intrahepatic cholangiocarcinoma cell line of a 61 year old Japanese woman. At first, it was mistakenly reported by Riken as a cell line contaminated with SSP-25, and therefore it was also reported by ICC as a cell line contaminated with SSP-25. But later it was discovered that SSP-25 was actually contaminated with ETK-1, not the other way around.
form	Epithelial like cells
Growth characteristics	Wall attached growth
Doubling time	2-3 times a week
STR	Amelogenin X CSF1PO 12 D5S818 10 D7S820 11 D13S317 12 D16S539 10,12 TH01 7 TPOX 8 vWA 14,17
culture conditions	Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37 degrees Celsius, humidity in the incubator is 70% -80%.
Freezing conditions	Cryopreservation solution: 90% FBS, DMSO 10%, Or use non programmed freezing solution: item number JY-H040
Product Usage	Due to scientific research limitations, it cannot be used as a therapeutic product for animal or human diseases.

3、 Brand Introduction

Shanghai Jinyuan Biotechnology Co., Ltd. is located in Shanghai and is a professional R&D enterprise that provides scientific research and medical technology services, focusing on molecular cloning, gene editing, recombinant proteins, cell services, and customization. Jinyuan Biotechnology provides full process services for cell culture, including primary cells, cell lines, cell strains, fetal bovine serum, serum-free cryopreservation solution, wanquan culture medium, basic culture medium, efficient mycoplasma removal agent, black fungus removal agent, efficient cell pollution removal agent, eukaryotic removal agent, bacterial removal agent, dual antibody and all experimental related products for cell culture. The company has a research and development team composed of professionals in the fields of cell biology, biochemistry, etc. They have rich experience and profound technical accumulation in the field of cell culture, and are able to continuously develop and optimize products to meet market demand and technological development. Jinyuan Biotechnology aims to provide customers with professional and comprehensive scientific research services. Adhering to the quality policy of scientific efficiency, independent standardization, rigorous practicality, accuracy and fairness, we provide better services to the vast number of biomedical research users with good reputation, excellent technology, and high-quality services!

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