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E-mail
2019632537@qq.com
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Phone
13752086572
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Address
B04032-36A-012, 4th Floor, Tianhong Building, Hongqiao District, Tianjin City
Tianjin Bairui Biotechnology Co., Ltd
2019632537@qq.com
13752086572
B04032-36A-012, 4th Floor, Tianhong Building, Hongqiao District, Tianjin City
1、 Product specifications
Product Code: JY-J1175
Product Name:Human esophageal squamous cell carcinoma KYSE-450 (STR identification correct)
Product specifications: 1 × 10 ⁶ cells/T25 culture bottle
Product brand: Jinyuan Biotechnology
2、 Product Introduction
Human esophageal squamous cell carcinoma KYSE-450 (STR identification correct)
genus |
person |
alias |
KYSE 450; KYSE450; Question450 |
Source of organization |
esophagus |
disease |
Esophageal squamous cell carcinoma |
Passage ratio/Cellular digestion |
1: 2 passages, digest for 2-3 minutes. |
Wanquan culture medium configuration |
RPMI1640 medium 44.5% and Ham's F-12 medium 44.5%; High quality fetal bovine serum, 10%; Double antibody 1% |
Introduction |
The cell was obtained from a patient before treatmentEstablishment of well differentiated invasive squamous cell carcinoma with mid thoracic esophagectomy in a 59 year old male (infiltration depth not exceeding submucosal layer);Described as carryingP53 mutation and amplification of oncogene MYC. |
form |
Epithelial like cells |
Growth characteristics |
Wall attached growth |
STR |
X CSF1PO Amelogenin 11,12 D2S1338 19,23 D3S1358 18 D5S818 10,13 D7S820 11,12 D8S1179 10 D13S317 11 D16S539 9 D18S51 13 D19S433 13,15 D21S11 30 FGA 24 Penta D 9,10 Penta E 11,16 TH01 8,9 TPOX 8 vWA 14 |
Doubling time |
weekly 2-3 times |
culture conditions |
Gas phase: Air ,95% Carbon dioxide ,5%. Temperature: 37 degrees Celsiusdegree The humidity of the incubator is70% -80%. |
Freezing conditions |
Freezing solution:90%FBS,DMSO 10%, Or use non programmed freezing solution: item numberJY-H040 |
Preservation institution |
DSMZ; ACC-387 |
Product Usage |
Limited to scientific research only and cannot be used as a therapeutic product for animal or human diseases. |
3、 Brand Introduction
Shanghai Jinyuan Biotechnology Co., Ltd. is located in Shanghai and is a high-tech research and development enterprise specializing in providing scientific research and medical technology services, focusing on molecular cloning, gene editing, recombinant proteins, cell services, and customization. Jinyuan Biotechnology provides full process services for cell culture, including primary cells, cell lines, cell strains, fetal bovine serum, serum-free cryopreservation solution, wanquan culture medium, basic culture medium, efficient mycoplasma removal agent, black fungus removal agent, efficient cell pollution removal agent, eukaryotic removal agent, bacterial removal agent, dual antibody and all experimental related products for cell culture. The company has a research and development team composed of professionals in the fields of cell biology, biochemistry, etc. They have rich experience and profound technical accumulation in the field of cell culture, and are able to continuously develop and optimize products to meet market demand and technological development. Jinyuan Biotechnology aims to provide customers with professional and comprehensive scientific research services. Adhering to the quality policy of scientific efficiency, independent standardization, rigorous practicality, accuracy and fairness, we provide better services to the vast number of biomedical research users with good reputation, excellent technology, and high-quality services!
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