Human dietary fiber (TDF) ELISA kit in stock

Human dietary fiber (TDF) ELISA kit in stock

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Overview of ELISA Kit Testing

ELISA (Enzyme Linked Immunosorbent Assay) is a highly sensitive experimental technique that combines the specific reactions of antigens and antibodies with the efficient catalytic activity of enzymes on substrates, based on immunological reactions. Due to the fact that the antigen and anti ti reactions are carried out in the wells of a solid-phase carrier - a polystyrene Z-ene microtiter plate, after incubation with each reagent added, excess free reactants can be removed by washing to ensure the specificity and stability of the test results. In practical applications, there can be multiple specific methods and steps through different designs. Namely, indirect methods for detecting anti ti, double antibody ti sandwich methods for detecting antigens, and antigen competition methods for detecting small molecule antigens or haptens, etc. At present, we provide customers with commonly used ELISA double antibody sandwich method and ELISA indirect method services.

Experimental process:

1. Reagent preparation: All reagents must reach room temperature before use, and please store them immediately according to the instructions after use. Please use disposable suction tips during experimental operations to avoid cross contamination.

2. Sample addition: When adding samples or reagents, please note that if the time interval between the first well and the last well is too large when taking samples/standards, enzyme conjugates, or substrates, it will result in different "pre incubation" times, which will significantly affect the accuracy and repeatability of measurement values. The time for adding samples (including standards and all samples) at once will be controlled within 10 minutes. If there are a large number of samples, it is recommended to use a multi pipette for sample addition.

3. Incubation: To prevent sample evaporation, the reaction plate is placed in a sealed box covered with a damp cloth during the experiment, and the enzyme-linked immunosorbent assay (ELISA) plate is covered or coated to prevent liquid evaporation; After washing the plate, the next step should be carried out as soon as possible, and at all times, the enzyme-linked immunosorbent assay plate should be avoided from being in a dry state. At the same time, the given incubation time and temperature should be strictly followed.

4. Washing: During the washing process, the residual washing solution in the reaction well should be thoroughly dried on the filter paper. Do not directly put the filter paper into the reaction well to absorb water. At the same time, eliminate the residual liquid and fingerprints at the bottom of the plate to avoid affecting the reading of the ELISA reader.

Precautions for operating ELISA kit:

1) Reagents should be stored according to the label instructions and returned to room temperature before use. The diluted standard should be discarded and cannot be stored.

2) The Flat noodles not used in the experiment shall be immediately put back into the packaging bag and sealed for storage to avoid deterioration.

3) Other unused reagents should be packaged or covered. Do not mix reagents of different batches. Use before shelf life.

4) Use disposable suction tips to avoid cross contamination, and avoid using sample dispensers with metal parts when suctioning termination solution and substrates A and B.

5) Use clean plastic containers to prepare detergent. Mix all components and samples in the reagent kit thoroughly before use.

6) When washing the enzyme-linked immunosorbent assay (ELISA) plate, it should be thoroughly dried. Do not directly put the absorbent paper into the ELISA reaction well to absorb water.

7) Substrate A should evaporate and avoid opening the lid for a long time. Substrate B is sensitive to light and should be avoided from prolonged exposure to light. Avoid contact with hands, toxic. After the experiment is completed, the OD value should be read immediately.

8) The order of adding reagents should be consistent to ensure that the incubation time for all reaction plate wells is the same.

9) Perform incubation operations according to the time, amount, and sequence indicated in the instructions.

Human dietary fiber (TDF) ELISA kit in stock

ELISA kit sample collection, processing, and storage methods:

1) Serum - Avoid any cellular stimulation during operation. Use test tubes without pyrogen and endotoxin. After collecting blood, centrifuge at 1000 × g for 10 minutes to quickly and carefully separate serum and red blood cells.

2) Plasma - EDTA, citrate, heparin plasma can be used for detection. Centrifuge at 1000 × g for 30 minutes to remove particles.

3) Cell supernatant - Centrifuge at 1000 × g for 10 minutes to remove particles and polymers.

4) Tissue homogenate - add an appropriate amount of physiological saline and crush the tissue. Centrifuge at 1000 × g for 10 minutes and collect the supernatant

5) Storage - If the sample is not used immediately, it should be divided into small parts and stored at -70 ℃ to avoid repeated freezing. Try not to use hemolysis as much as possible. If there are a large number of particles in the serum, centrifuge or filter before detection. Do not thaw by heating at 37 ℃ or higher.

Storage and expiration date of reagent kit:

Unopened reagent kit: All reagents are stored as indicated on the label of the reagent bottle. Please note that upon receipt of the reagent kit, please store the TMB washing solution and termination solution at 4 ℃ as soon as possible, and store other reagents at -20 ℃.

After use reagent kit: The remaining reagents should still be stored at the temperature indicated on the label of the reagent bottle. The opened enzyme-linked immunosorbent assay (ELISA) plate should be sealed with a desiccant and stored at -20 ℃ to avoid moisture.

Stability: After testing, the reagent kit has been stored at the recommended temperature within its validity period, and its activity reduction rate is less than 5%. To reduce the influence of external factors on the detection values before and after the destruction of the reagent kit, the laboratory environment conditions should be kept as consistent as possible, especially the temperature, humidity, and incubation conditions inside the laboratory. Secondly, having the same experimenter perform the operation can reduce human error.

Disclaimers

The reagent kit is for research purposes only and cannot be used for clinical or human experiments. Any consequences arising from this shall be borne by the experimenter, and our company shall not be held responsible.

Strictly follow the instructions for operation. If the experimenter violates the instructions, the consequences shall be borne by the experimenter.

[Explanation]

Due to the current conditions and scientific and technological level, it is not yet possible to comprehensively identify and analyze all raw materials provided by all suppliers, which may pose certain quality and technical risks to this product.

2. The final experimental results are closely related to the effectiveness of the reagents, the relevant operations of the experimenter, and the experimental environment at that time. Please be sure to prepare sufficient sample backups.

3. There may be slight differences in the detection limit, sensitivity, and color development time of the same product from different batches. Please refer to the instructions in the reagent kit for experimental operations. The electronic version of the instructions on the website is for reference only.

4. Only by using all the reagents in this kit can the detection effect be guaranteed, and products from other manufacturers cannot be mixed. Only by strictly following the experimental instructions of this kit can the test results be obtained.

5. Our company is only responsible for the reagent kit itself and is not responsible for the sample consumption caused by the use of the kit. Users are advised to fully consider the possible usage of the sample and reserve sufficient samples before use.

6. Tissue homogenates or cell extracts prepared using chemical lysates may result in biased ELISA experimental results due to the introduction of certain chemicals.

7. If the sample is a cell culture supernatant, there may be many interfering factors such as cell status, cell quantity, sampling time, etc., so there may be situations where it cannot be detected.

8. Some natural or recombinant proteins, including prokaryotic and eukaryotic recombinant proteins, may not be detected due to their mismatch with the detection and capture antibodies used in this product.

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