Human complete cobalamin 2 (HTSB2) ELISA kit in stock

Human complete cobalamin 2 (HTSB2) ELISA kit in stock

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ELISAFa is a new technology in immunodiagnosis, which has been successfully applied to immunodiagnosis of infectious diseases, parasitic diseases, and non infectious diseases caused by various pathogenic microorganisms. It has also been applied to the quantitative determination of large molecule antigens and small molecule antigens. Based on the results already used, it is believed thatELISAThe method has the characteristics of sensitivity, specificity, simplicity, speed, stability, and ease of automated operation. Not only suitable for the examination of clinical specimens, but also because hundreds or even thousands of specimens can be examined within a day,Therefore, it is also suitable forSerum epidemiologyinvestigation This method can not only be used to determineresisttiMoreover, it can also be used to determine circulating antigens in body fluids, making it a good method for early diagnosis. ThereforeELISAThe application scope of law in various fields of biomedical science is expanding day by day,

composition structure

1Serum: Avoid any cellular stimulation during the operation. Use without pyrogen and internal componentsduThe vegetarian test tube. After collecting blood,Centrifuge at 1000 × g for 10 minutesRed blood cellsQuickly and carefully separate.

22. Plasma: EDTA, LemonsuanSalt and heparin plasma can be used for detection.Centrifuge at 1000 × g for 30 minutes to remove particles.

3. Cell supernatant: Centrifuge at 1000 × g for 10 minutes to remove particles and polymers.

4. Tissue homogenate: Crush the tissue by adding an appropriate amount of physiological saline. Centrifuge at 1000 × g for 10 minutes and collect the supernatant.

5. Storage: If the sample is not used immediately, it should be divided into small parts and stored at -70 ℃ to avoid repeated freezing. Try not to use hemolysis as much as possible. If there are a large number of particles in the serum, centrifuge or filter before detection. Do not thaw by heating at 37 ℃ or higher. Thaw at room temperature and ensure that the sample is evenly thawed.

Human complete cobalamin 2 (HTSB2) ELISA kit in stock

Operation steps:

1Before use, mix all reagents thoroughly. Do not make the liquid produce a lot of foam, so as not to add a lot of bubbles when adding samples, which will cause errors in adding samples.

2. Determine the number of Flat noodles required according to the number of samples to be tested plus the number of standards. It is recommended to make duplicate holes for each standard and blank hole. Each sample is determined according to its own quantity, and those that can be reused should be reused as much as possible.

3. Add 50ul of diluted standard substance to the reaction well and 50ul of the test sample to the reaction well. Immediately add 50ul of labeled antibody. Cover the membrane plate, gently shake and mix well, and incubate at 37 ℃ for 45 minutes.

4. Shake off the liquid in the holes, fill each hole with detergent, shake for 30 seconds, shake off the detergent, and pat dry with absorbent paper. Repeat this operation 4 times. If washing with a washing machine, increase the number of washes by one.

5. Add 100ul of affinity chain enzyme HRP to each well, gently shake and mix well, and incubate at 37 ℃ for 30 minutes.

6. Shake off the liquid in the holes, fill each hole with detergent, shake for 30 seconds, shake off the detergent, and pat dry with absorbent paper. Repeat this operation 4 times. If washing with a washing machine, increase the number of washes by one.

7. Add 50ul of substrate A and B to each well, gently shake and mix well, and incubate at 37 ℃ for 5 minutes. Avoid exposure to light.

8. Take out the enzyme-linked immunosorbent assay (ELISA) plate and quickly add 50ul of termination solution. The results should be measured immediately after adding the termination solution.

9. Measure the OD values of each well at a wavelength of 450nm.

Precautions for operation:

1Reagents should be stored according to the label instructions and returned to room temperature before use. The diluted standard should be discarded and cannot be stored

2The Flat noodles not used in the experiment shall be immediately put back into the packaging bag and sealed for storage to avoid deterioration.

3Other unused reagents should be packaged or covered. Do not mix reagents of different batches. Use before shelf life.

4Use disposable suction tips to avoid cross contamination and extract termination solution and substrateA. When using liquid B, avoid using a sampler with metal parts.

5Use clean plastic containers to prepare detergent. Mix all components and samples in the reagent kit thoroughly before use.

6substrateA should evaporate and avoid opening the lid for a long time. Substrate B is sensitive to light and should be avoided from prolonged exposure to light. Avoid contact with hands, toxic. After the experiment is completed, the OD value should be read immediately.

7. The order of adding reagents should be consistent to ensure that the incubation time for all reaction plate wells is the same. Perform incubation operations according to the time, amount, and sequence indicated in the instructions.

Generally speaking,96T can detect 90 samples, and 48T can detect 42 samples. Standard samples are needed to make the standard curve. Therefore, teachers who need to do experiments, please pay attention to how many samples need to be made when purchasing ELISA kits, and decide on the size of the kit to avoid insufficient or wasted kits during experiments!

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