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Shanghai Qiansi Biotechnology Co., Ltd
Human cartilage oligomeric protein kit
NegotiableUpdate on 05/06
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Overview
The human cartilage oligomeric protein kit, using ELISA technology, is highly sensitive, specific, ultra stable, and easy to operate. Assist in scientific research and clinical practice, used to detect the protein in samples, and operate according to procedures to ensure reliable results.
Product Details
Human cartilage oligomeric protein kit
Human cartilage oligomeric protein kitAs an important tool in the field of medical testing, it provides strong support for in-depth exploration of the physiological and pathological mechanisms related to human cartilage and clinical disease diagnosis.
Detection PrincipleEnzyme linked immunosorbent assay (ELISA) technology is commonly used. The microplate in the reagent kit is pre coated with specific antibodies against human cartilage oligomeric proteins. After adding the test sample, the cartilage oligomeric protein in the sample will specifically bind to the solid-phase antibody, forming an antigen antibody complex. Subsequently, enzyme-linked immunosorbent assay (ELISA) secondary antibodies were added, which further combined with the already bound antigen antibody complex to construct a stable "solid-phase antibody cartilage oligomeric protein ELISA secondary antibody" structure. After adding the substrate, under the catalytic action of the enzyme, the substrate undergoes a color reaction, and the depth of the color is positively correlated with the content of cartilage oligomeric proteins in the sample. Researchers or medical staff can use an enzyme-linked immunosorbent assay (ELISA) reader to measure absorbance at specific wavelengths and compare it with the standard curve provided by the kit to accurately calculate the concentration of cartilage oligomeric proteins in the sample.
Product FeaturesThis kit has high sensitivity and can sensitively detect extremely small amounts of cartilage oligomeric protein. Even in the early stages of the disease, if the protein content in the body only changes slightly, it can be accurately captured. The specificity is excellent, and the antibodies have been rigorously screened and optimized to only produce specific reactions to cartilage oligomeric proteins, effectively avoiding cross reactivity with other similar proteins and ensuring accurate and reliable detection results. In terms of stability, it is equally excellent. The strict production process and quality control system ensure that the performance of the reagent kit remains stable within the validity period under the specified storage conditions of 2-8 ℃, and the test results between different batches have good consistency. Moreover, the operation is relatively simple, the reagent kit comes with detailed instructions, the reagent components are complete, and personnel who have received simple training can operate according to the standardized procedures.
Scope of applicationIn the field of scientific research, it helps researchers to deeply analyze the expression changes of cartilage oligomeric proteins in normal physiological processes and various joint disease pathological states, providing key clues for revealing the pathogenesis of diseases. In clinical diagnosis, it has important auxiliary diagnostic value. For example, in patients with osteoarthritis, the level of cartilage oligomeric protein in serum or joint fluid often increases. Testing this indicator helps doctors evaluate the severity and progression of the disease. In the research and diagnosis of other joint diseases, such as rheumatoid arthritis, the detection results of cartilage oligomeric proteins can also provide important references for clinical decision-making.
operating procedureWhen using, the first step is to collect samples in a standardized manner. Common samples include serum, plasma, and joint fluid. During the collection process, attention should be paid to avoiding sample contamination and hemolysis. Timely process the sample after collection, such as centrifuging serum or plasma. Prepare various reagents in the kit and equilibrate them to room temperature. Then, the steps of sample addition (including standard and sample), incubation (to fully react the antigen and antibody), washing (to remove unbound substances), adding enzyme-linked secondary antibody, re incubation, washing, adding substrate for color development, and terminating the reaction are carried out in sequence. Finally, use an enzyme-linked immunosorbent assay (ELISA) reader to read the absorbance values at a specific wavelength, and calculate the concentration of cartilage oligomeric proteins in the sample based on the standard curve. The entire operation process must be strictly carried out in accordance with the instructions to ensure the reliability of the test results.
