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Human breast cancer susceptibility protein 2 kit
NegotiableUpdate on 05/06
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Overview
BRCA2 in human breast cancer susceptibility protein 2 kit is detected by Duoyi ELISA. It has high sensitivity, strong specificity, good stability, and simple operation, which helps scientific research and clinical screening and assessment of breast cancer risk.
Product Details
Human breast cancer susceptibility protein 2 kit
Human breast cancer susceptibility protein 2 kitIt is a detection tool of great significance in the field of breast cancer research and clinical diagnosis, mainly used to accurately determine the content of breast cancer susceptibility protein 2 (BRCA2) in human samples.
Detection PrincipleThe common detection principle is enzyme-linked immunosorbent assay (ELISA). In the reagent kit, the specific antibody against BRCA2 is coated on a microplate to prepare a solid-phase antibody. After adding the sample, BRCA2 in the sample will bind to solid-phase antibodies, followed by the addition of enzyme-linked secondary antibodies to form antibody antigen enzyme-linked antibody complexes. After thorough washing, the substrate TMB was added for color development. TMB is converted to blue by enzyme catalysis and to the final yellow by acid action. The depth of color is positively correlated with the BRCA2 content in the sample. Researchers measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of BRCA2 in the sample based on the standard curve.
Product FeaturesIn terms of sensitivity, it shows excellent performance and can detect extremely low levels of BRCA2. Even if the content in the sample is extremely small, it can be accurately detected, which helps to discover subtle changes in BRCA2 in early disease states. Strong specificity, the high specificity antibodies used can accurately recognize BRCA2, effectively avoiding cross reactivity with other related proteins or impurities, providing a solid guarantee for the accuracy of experimental data. At the same time, it is also reliable in terms of stability, with strict production processes and quality control to ensure that the performance of the reagent kit remains stable within the validity period under the specified storage conditions of 2-8 ℃, and the test results between different batches have good consistency. The operation process is relatively simple, and the reagent kit is equipped with detailed instructions. Operators can operate accurately after certain training.
Scope of application: It is mainly used in scientific research to help researchers deeply explore the role of BRCA2 in normal physiological processes and the pathogenesis of breast cancer. For example, when studying familial hereditary breast cancer, we can analyze the relationship between BRCA2 and the risk of breast cancer by detecting the change of BRCA2 content. In terms of clinical diagnosis, BRCA2 detection is carried out for high-risk groups such as breast cancer family history and abnormal breast tissue, which is helpful for early screening of breast cancer, assessment of disease risk, and provides basis for the formulation of personalized prevention and treatment plans.
operating procedureTaking ELISA kit as an example, first collect suitable samples such as blood, tissue homogenate, etc. When collecting serum samples, use test tubes without pyrogen and endotoxin, centrifuge at 3000 rpm for 10 minutes, and then separate the serum. After collecting the samples, a series of steps are carried out, including dilution and addition of standard samples, incubation, washing, enzyme addition, color development, and reaction termination. During the operation, it is necessary to strictly follow the instructions, control the reaction time, temperature and other conditions, and finally read the OD value on the enzyme-linked immunosorbent assay reader and calculate the result. Although the entire operation process is not complicated, each step is crucial and needs to be treated rigorously to ensure the reliability of the test results.
