Human breast cancer susceptibility protein 1 kit

Human breast cancer susceptibility protein 1 kit

Shanghai Qiansi Biotechnology Co., LtdHuman breast cancer susceptibility protein 1 kitThere are nearly 3000 kinds of cells, which come from ATCC in the United States. The cells are all under strict quality control and strive to build a bridge between authentic cells and domestic researchers in Chinese Mainland. In addition to providing you with more related experimental products, standards, cell lines, and experimental technology services, we also offer pre - and post service support. Choosing the right experimental reagents or materials is the key to successful experiments

Notice on Purchasing Cells from Shanghai Qiansi Biotechnology (Qiansi Biotechnology)

1、 The customer does not open the lid when receiving the cells, and leaves them in the incubator for several hours (depending on the cell density) to observe the growth of the cells under an inverted microscope, and takes photos of the cells at different magnifications (it is recommended to take a photo of the culture medium when receiving the cells, observe the color of the culture medium and whether there is any leakage, and take one photo of the cells at 100X and 200X under the microscope), to eliminate contamination of the cells themselves;

We are responsible for sending a free cell line in case of contamination due to unopened cells received.

2、 If it is an adherent cell, please observe the cell density under a microscope:

1. When the confluence degree does not exceed 80%, use 75% alcohol (it is recommended to prepare water with 75% alcohol, which is also sterilized ultrapure water), spray the entire bottle for disinfection, and place it in the sterilization table for strict aseptic operation; then open the lid, first suck out about 10ml of the culture medium, place it in the centrifuge tube, and then burn the bottle mouth (it is strictly prohibited to pour it directly) to ensure no contamination. Then use a 10ml pipette to suck out the remaining culture solution and place it in the centrifuge tube. Only about 10ml of culture solution is left in the culture bottle. After 16 hours of cultivation (time adjusted according to cell growth), passage or cryopreservation.

When the confluence exceeds 80%, please subculture according to the cell culture conditions. The specific steps are as follows:

1) Discard the culture medium and wash 1-2 times with 3ml PBS (without calcium or magnesium ions);

2) Add 1ml of digestion solution (0.25% Trypsin 0.53mM EDTA) to a T25 culture bottle, invert and place in a 37 ° C incubator for 1-3 minutes to preheat. Then invert the culture bottle for about 30 seconds and observe the cell digestion under an inverted microscope. If most of the cells become round and dispersed, tap the culture bottle lightly and the cells will immediately fall off;

3) Add 6-8ml of culture medium, aspirate, and transfer to new culture bottles as required.

3、 If it is a suspended cell, aspirate the culture medium, centrifuge at 1000 rpm for 5 minutes, aspirate the supernatant, suspend the cells in fresh culture medium at the bottom of the tube, and transfer them back to the culture bottle.

Attention: When changing the medium, use half of our culture medium and half of yours to avoid poor growth caused by cell adaptation.

4、 What are the situations where cells have problems and can be resent? What are the criteria for judgment?

(1) Various problems encountered during cell transportation, such as cell loss, damage, leakage, etc., and resending;

(2) After the recovery of frozen cells, the vast majority of cells did not survive and reappeared;

(3) After 24 hours of standing, the vast majority of surviving cells did not survive and reappeared;

(4) After thawing frozen cells or allowing surviving cells to stand for 4 hours without opening, if contamination occurs, resend;

(5) It depends on the specific situation.

Acris abcam cst Biorbyt santa Novus sigma lifespan NEB roche ABI R&D millipore BD Qiagen Cayman Jackson Life GeneTex Bio-Rad DSHB tocris peprotech Waiting for brands; Some products are in stock at ultra-low prices, and we also have Trizol DMSO lipo2000 lipo3000 SC-2004 SC-2005 in stock; Short delivery time, competitive price, and comprehensive after-sales service.

Three principles of cell culture technology: 1. Use your own set of reagents and do not mix them with others; 2. Observe cells diligently, love and care for them like keeping a pet; 3. Regularly change the liquid and passage, do not "fish for three days, sun screen for two days".
Reliable quality and guaranteed after-sales service

Our bank has nearly 3000 types of cells, sourced from ATCC in the United States, all of which have undergone strict quality control.

The freezing time for cells is 5-7 working days, and for live cells it is 7-15 working days.

Due to the large number of cell categories in the cell bank, in order to avoid confusion and errors, we need to know the relevant cell prices and detailed information. Please forgive any inconvenience caused to you!

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