Human breast cancer marker - CA153 kit

NegotiableUpdate on 05/06

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Overview

The human breast cancer marker - CA153 kit uses ELISA double antibody sandwich method to quantitatively measure the sample CA153. Through sample collection, sampling and other operations, it has high sensitivity and specificity, and is used for early screening, disease monitoring and efficacy evaluation of breast cancer.

Product Details

Human breast cancer marker - CA153 kitIntroduction

Human breast cancer marker - CA153 kitIt is an important tool for quantitative detection of CA153 content in human serum, plasma and other samples in vitro, and plays a key role in the diagnosis and treatment of breast cancer.

Detection Principle

This kit is based on enzyme-linked immunosorbent assay (ELISA) technology and uses a double antibody sandwich method. The microplate in the reagent kit is pre coated with specific antibodies against CA153. When adding the test sample, CA153 in the sample will specifically bind to the coated antibody. Subsequently, enzyme labeled anti-CA153 antibody is added, which further binds to CA153 already bound to the coated antibody, forming a "coated antibody CA153 enzyme labeled antibody" complex. After adding the substrate solution, under the catalytic action of the enzyme, the substrate undergoes a color reaction, and the depth of the color is proportional to the content of CA153 in the sample. By measuring the absorbance (OD value) at a specific wavelength using an enzyme-linked immunosorbent assay (ELISA) reader, the content of CA153 in the sample can be accurately calculated based on a pre drawn standard curve.

Usage

sample collectionAccording to the testing requirements, collect an appropriate amount of human serum or plasma samples. Serum samples need to be obtained by centrifugation after natural coagulation of the blood; Plasma samples need to be centrifuged and separated after adding appropriate anticoagulants during blood collection. The collected samples should be properly stored to avoid repeated freezing and thawing, in order to prevent any impact on the test results.

Preparation of reagent kitTake out the reagent kit from the refrigerator and equilibrate at room temperature (20 ℃ -25 ℃) for 30 minutes to ensure that the temperature of each reagent inside the kit is consistent. At the same time, prepare experimental equipment such as pipettes, pipettes, enzyme-linked immunosorbent assay (ELISA) reader, etc. to ensure their good performance.

sample additionAccurately add the standard sample, test sample, and blank control to the corresponding wells of the microplate according to the specified volume, gently shake and mix to ensure full contact between the sample and the coated antibody, and then incubate at the set temperature for a certain period of time.

PCB cleaningAfter incubation, discard the liquid in the well and thoroughly wash the microplate 3-5 times with washing solution to completely remove unbound substances and reduce non-specific signal interference. After each wash, the microplate should be dried to prevent residual liquid from affecting subsequent reactions.

Add enzyme labeled antibodyAdd an appropriate amount of enzyme-linked antibody to each well, shake and mix well, and then incubate again.

Color rendering and terminationAdd substrate chromogenic solution and incubate under dark conditions. When the color changes to an appropriate degree, add termination solution to stop the reaction and bring it to a good detection state.

Result determinationImmediately use an enzyme-linked immunosorbent assay (ELISA) reader to measure the OD values of each well at the specified wavelength, and calculate the content of CA153 in the sample based on the standard curve.

Performance characteristics

high sensitivityCapable of detecting extremely low concentrations of CA153, meeting the detection requirements for trace samples or low expression levels. For example, changes in CA153 levels at the Pick level can be accurately detected.

Strong specificityThe antibodies in the kit have high specificity for CA153 and minimal cross reactivity with other similar substances, ensuring the accuracy of the test results. Can accurately identify and determine CA153 in complex biological samples.

Good repeatabilityThe results of multiple tests on the same batch of samples are stable with a small coefficient of variation, providing reliable and reproducible data support for scientific research and clinical diagnosis. Repeatedly testing the same batch of samples multiple times results in minimal deviation.

Application scenarios

Early screening of breast cancer: To help preliminarily screen out individuals who may suffer from breast cancer in the population, and to improve the early diagnosis rate in combination with other inspection methods.

disease surveillance: For patients with confirmed breast cancer, the content of CA153 is regularly detected to track the development of the disease and evaluate whether the tumor is metastatic or recurrent.

efficacy evaluation: During the treatment of breast cancer, such as after surgery, hua therapy and radiotherapy, detect the change of CA153 content, judge whether the treatment plan is effective, and provide a basis for subsequent treatment strategy adjustment.