Human atrial fibroblasts

Human atrial fibroblasts

personatriumFibroblasts isolated fromThe atrial tissue of the heartHuman atrial fibroblasts are a key resident stromal cell in the atrial tissue of the heart. They are far from simple structural fillers, but have multiple dynamic and complex functions, playing a crucial role in the structural integrity, electrophysiological stability, signal transduction, and pathological remodeling of the atriumFibroblasts are relatively large, with clear contours and mostly flat structures resembling spindle or star shaped protrusions. Their nuclei are regular oval shaped, and their nucleoli are large and distinct. Fibroblasts have strong functional activity, weak alkaline cytoplasm, and significant protein synthesis and secretion activities. Under certain conditions, they can achieve interconversion with fibroblasts; Fibroblasts play a crucial role in the repair of varying degrees of cellular degeneration, necrosis, and tissue defects. The newly isolated embryonic lung fibroblasts are round in shape and have good refractive index, suspended in the culture medium.30minCells adhere to the wall, and some of them begin to protrude pseudopodia, manifested as small protrusions;6hThe posterior cells are basically adhered to the wall, stretched into a spindle shape, with clear nuclei, evenly distributed, scattered growth, and do not aggregate into clusters; Cells grow rapidly,5-7The sky is in a fused state, with tightly arranged cells, some overlapping and growing, flat, and larger cell bodies. The cytoplasm is transparent, and the nucleus is large, oval shaped, and light in color. Cells fuse and connect with each other to form a network; The cells are distributed in a flat spindle or star shape with protrusions. The cells are distributed in a flat spindle or star shape with protrusions. This cell plays an important role in synthesizing and secreting cytokines, maintaining a dynamic balance between inside and outside blood vessels, and coagulation and fibrinolysis. Human atrial fibroblasts are key regulators of the atrial microenvironment, and their functions are achieved through multiple pathways such as extracellular matrix metabolism, signal transduction, inflammatory response, and mechanical perception. Under normal physiological conditions, fibroblasts maintain the homeostasis of atrial structure and function; Under pathological conditions, its abnormal activation is one of the core driving factors for atrial fibrosis and arrhythmia.

Method Introduction:

Human atrial fibroblasts are treated with collagenase-Prepared by trypsin combined with digestion and differential adhesion method, with a total cell count of approximately5×10⁵cells/Bottle.

quality inspection：

Human atrial fibroblast cellsVimentinImmunofluorescence identification, purity can reach90%Above, and not containingHIV-1TheHBVTheHCVMycoplasma, bacteria, yeast, fungi, etc.

cultivateNutrition information:

Shanghai Yuchun Biotechnology Co., Ltd. is a technology enterprise jointly founded by personnel with excellent backgrounds in life sciences and biotechnology. The company focuses on primary cells, tumor cells, and cell culture related reagent products, providing technical support and services for universities, food, medicine, biological research, and other fields.

Since its establishment, the company has provided customized cell products and complete cell culture solutions to customers through continuous technological innovation and product research and development. Through active exploration, research and development, deepening and enriching of products, there are currently more than 200 types of basic culture medium products, more than 300 cell lines, and nearly 600 types of primary cells. We always take "developing science and technology, caring for public life and health" as our driving force. Through technological innovation, we extensively attract high-tech talents, continuously improve our product system and enrich our product chain, actively expand our market and application areas, and establish a competitive "Yuchun" brand in the market.

Precautions

1. For your safety and health, please wear lab coats and disposable gloves and masks when operating;

2. To maintain the excellent performance of this product, please be sure to store it according to the recommended storage conditions;

3. This product is only for scientific research or further production use and cannot be used for clinical diagnosis or treatment.

Human atrial fibroblasts