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Shanghai Qiansi Biotechnology Co., Ltd
Human Tryptophan tRNA Synthase Kit
NegotiableUpdate on 05/06
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Overview
The human tryptophan tRNA synthetase kit is used to detect the content of WARS in serum and other samples. The ELISA method is sensitive, specific, and stable, suitable for scientific research. It is easy to operate but requires standardization.
Product Details
Human Tryptophan tRNA Synthase Kit
Human Tryptophan tRNA Synthase Kit, is a widely used detection tool in the field of scientific research, mainly used for precise determination of the content of tryptophan tRNA synthetase (WARS) in human serum, plasma, and related liquid samples.
The core detection principle is the double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Firstly, the purified human tryptophan tRNA synthetase antibody is coated onto a microplate to prepare a solid-phase antibody. After adding the sample, the tryptophan tRNA synthetase will bind to the solid-phase antibody, and then bind to the HRP labeled tryptophan tRNA synthetase antibody, forming an antibody antigen enzyme labeled antibody complex. After thorough washing, the substrate TMB was added for color development. TMB is converted to blue under the catalysis of HRP enzyme and ultimately turns yellow under the action of acid. The depth of color is positively correlated with the content of tryptophan tRNA synthetase in the sample. Researchers measure the absorbance (OD value) at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the concentration of tryptophan tRNA synthetase in the sample based on the standard curve.
This reagent kit has many significant advantages. In terms of sensitivity, it performs excellently and can detect extremely low concentrations of WARS. Even if the content in the sample is extremely small, it can accurately capture and help discover subtle changes in biological processes. It has strong specificity, and the high specificity antibodies used can accurately recognize WARS, effectively avoiding cross reactions with other related proteins or impurities, providing a solid guarantee for the accuracy of experimental data. At the same time, it is also reliable in terms of stability, with strict production processes and quality control to ensure that the performance of the reagent kit remains stable within the validity period under the specified storage conditions of 2-8 ℃, and the test results between different batches have good consistency.
From the perspective of applicability, it mainly focuses on the field of scientific research. For example, when studying the mechanism of protein synthesis in cells, changes in the content of WARS may affect the synthesis of specific proteins, and this kit can be used to explore this process in depth. Moreover, in some disease-related studies, such as rare or metabolic diseases, the expression level of WARS may be abnormal, and the kit can help researchers understand its association with disease occurrence and development.
In terms of operation process, the reagent kit is equipped with detailed instructions. Firstly, sample collection can be conducted, including serum, plasma, and cell supernatant. Taking serum as an example, it is necessary to collect blood in a test tube without pyrogen and endotoxin, centrifuge at 3000 rpm for 10 minutes, and then separate the serum. After collecting the samples, a series of steps including dilution and addition of standard samples, incubation, washing, enzyme addition, color development, and reaction termination are carried out. Finally, the OD value is read on the enzyme-linked immunosorbent assay reader and the results are calculated. The operation process is relatively simple, but it is necessary to strictly follow the instructions to ensure the reliability of the test results.
