Highly active recombinant human EGF

Highly active recombinant human EGF

The human epidermal growth factor (EGF) is a milestone discovery in the field of life sciences, and its research has greatly promoted the development of cell biology, oncology, and regenerative medicine.

1、 The discovery process of EGF

The discovery story of EGF began in the 1960s.

▪ In 1962, American scientist Stanley Cohen accidentally discovered that another protein extracted from the submandibular gland of mice could significantly promote premature eye opening and tooth growth in newborn mice while studying nerve growth factor (NGF).

▪In 1972, Cohen's team successfully isolated and purified this protein, named "epidermal growth factor", because it can directly stimulate the growth and proliferation of epidermal cells.

▪In 1986, Stanley Cohen and Rita Levi Montalcini (for their research on NGF) were jointly awarded the Nobel Prize in Physiology or Medicine, in recognition of their pioneering work in the field of growth factors.

▪Subsequent breakthroughs:

• The human EGF gene was cloned in 1984.

• The EGF receptor (EGFR) was discovered in 1980, and its structure is highly homologous to the oncogene v-Orb, revealing the deep connection between growth factors and cancer.

2、 Biological functions of EGF

EGF is a small molecule peptide composed of 53 amino acids, which functions by binding and activating on the cell membraneThe epidermal growth factor receptor (EGFR) is activated.

Core mechanism of action:

1. Binding receptor: EGF binds to the extracellular domain of EGFR, inducing receptor dimerization.

2. Activation of signaling pathways: The intracellular tyrosine kinase of the receptor is activated, triggering multiple downstream signaling pathways (such as RAS-RAF-MAPK, PI3K-AKT, etc.).

3. Regulating gene expression: ultimately affecting cell cycle, metabolism, morphology, etc., promoting cell proliferation, differentiation, and survival.

Main biological functions:

▪Promote cell proliferation and differentiation: directly stimulate the proliferation of epidermal cells, keratinocytes, fibroblasts, etc., accelerating tissue repair.

▪Inhibition of cell apoptosis: Maintaining cell survival by activating anti apoptotic signals.

▪Participate in embryonic development: play a crucial role in embryo formation and organ development.

▪Regulating other physiological processes: affecting gastric acid secretion, hair follicle development, etc.

3、 Application areas of EGF

1. Clinical Medicine and Regenerative Medicine

▪Trauma repair:

• Burns, ulcers, diabetes feet: EGF ointment (such as our "Kangfuxin" series products) can significantly accelerate wound healing and reduce scars.

• Corneal injury: Eye drops are used for corneal transplantation and repair after chemical burns.

▪Surgical procedure: Spraying EGF solution during surgery promotes tissue healing and reduces complications.

▪Digestive system diseases: Oral EGF is used for repairing gastric and duodenal ulcers (due to its acid resistance and ability to be absorbed by the intestine).

2. Targeted therapy for tumors

▪EGFR as a target: Many tumors (such as non-small cell lung cancer, colorectal cancer, head and neck cancer) have EGFR overexpression or mutation.

▪Targeted drugs:

• Monoclonal antibodies: cetuximab, panimab, block EGFR signaling.

• Small molecule inhibitors: gefitinib, erlotinib, inhibit tyrosine kinase activity.

▪Accompanying diagnosis: Detecting tumor EGFR mutation status and guiding medication.

3. Skin care and cosmetics

▪Anti aging and repair: Skincare products containing EGF claim to promote epidermal renewal, increase collagen, and reduce wrinkles.

▪Disputes and Regulation:

• Effectiveness: There is controversy over whether topical EGF can penetrate the stratum corneum and maintain its activity.

• Safety: There is concern about whether long-term use stimulates the growth of potential cancer cells (the European Union has restricted the use of EGF in cosmetics).

4. Biotechnology and Scientific Research

▪Cell culture: As a key additive, it is used to maintain the in vitro proliferation of stem cells and primary cells.

▪Tissue engineering: Combining with biomaterials to construct artificial skin, cornea, etc.

▪Signal transduction research: As a classic model, it is used to study cell communication and cancer mechanisms.

Serruicheng provides highly active recombinant human epidermal growth factor (EGF)

The highly active recombinant human epidermal growth factor (EGF) provided by Serruicheng is prepared, inspected, and packaged in a clean room that meets GMP standards.

Purity: purity>95% as detected by SDS-PAGE electrophoresis;

Biological activity: ED50 less than 2 ng/ml was determined by dose-dependent proliferation assay using 3T3 cells;

Endotoxin: less than 1 EU/μ g.

The highly active recombinant human epidermal growth factor (EGF) products from Serruicheng have been widely used in cell culture related research and industrial production fields, and have gained recognition from a large number of customers.

Order Information