High throughput microbioreactor

BioLector II high-throughput microreactor

BioLector IIIt is a new type of micro bioreactor that is robust and easy to operate. This system can perform multiple high-throughput fermentations repeatedly and can measure various common fermentation parameters in real-time, such as biomass and acidity/alkalinity(pH)Dissolved oxygen value(DO)And fluorescent molecules). After multiple practices, it has been proven that this miniature bioreactorBioLectorIt has been successfully applied in the biotechnology industry, and many customers even purchase multiple systems.
This system is designed for use with standard microplates（MTP）Micro fermentation with non-invasive optical sensors for operation. During optical measurement and throughout the entire experimental phase, it is highly suitable for microbial fermentation due to its ability to maintain continuous and rigorous vibration. Moreover, this micro bioreactor can achieve large-scale parallel and real-time monitoring of aerobic and anaerobic cultivation processes!

system
BioLector II / ProMicro bioreactors, specially developed for bacteria, yeast, fungi, plants, and insect cells, are unique and suitable for
High throughput fermentation system with aerobic, micro aerobic, and strict anaerobic cultivation. Can perform up to simultaneously48Parallel cultivation and online monitoring of biomass concentrationpHValueDOAnd basic fermentation parameters such as fluorescent proteins or substrates. of whichBioLector ProA specialized microplate using advanced microfluidic chips that can continuously control the growth of each culture wellpHValue and replenishment.

automation
RoboLectorsupportBioLectorHigh throughput fermentation technology, online monitoring capability, and precise and repeatable pipetting integrated with automated pipetting systems. Provided customers with a unique automated training platform. It can be used for culture medium preparation, automatic sampling and addition, induction experiments, and feeding research.

Consumables
m2p-labsProvide unique microporous plates——48Kongmeihua board and circular hole board, when used in conjunction with different optical sensors, are suitable for different applications.FlowerPlatePlum blossom hole design can provide sufficient oxygen to meet the needs of high aerobic cultivation. Circular hole plates are suitable for organisms with low oxygen demand or highly sensitive to shear stress. In addition, microfluidic technology supports32 Online feeding and replenishment in a cultivation wellpHControl.

application
•Screening of bacterial strains and cell lines
•Screening and optimization of culture medium
•Fermentation parameter optimization
•Anaerobic fermentation and microaerophilic fermentation
•Synthetic Biology and Systems Biology
•Experimental Design（DoE）
•Growth characteristic analysis
•Protein Structure Dynamics
•High throughput protein expression
•Enzyme and cell activity testing
•functional genomics
•Proteomic research
•Growth inhibition and toxicity testing
•quality control

Online parameters
•biomass concentration
•pHprice
•dissolved oxygen value(DO)
•NAD(P)HAnd riboflavin
•Fluorescent molecules (green fluorescent protein, yellow fluorescent proteinRed fluorescent protein, etc

performance
•The working volume is800–2400μL
•48 A parallel microreactor
•kLaWide range of values(25–600 1/h)
•Continuous gas exchange and oxygen supply
•The input power of each reactor is equal
•Established process parameters and scalability
•Gas environment control（CO2，O2andN2）
•Humidity control(> 75% rH)
•Temperature control with active cooling function (lower than room temperature)5℃）

advantage
•answer48Real time dynamic monitoring of parallel fermentation
•Standard microporous plate(MTP)Micro fermentation of formula
•Combined with automated pipetting systems, it can achieve:
•Batch and feeding batch fermentation
•Preparation of culture medium
•High throughput and automation
•Wide range of biomass detection
(The highest equivalent to250 OD600，50g/L CDW，
Using Escherichia coli as the measurement object
•Biomass detection can be performed online without dilution
•lowpHValue measurement range3.6-6.5
•Small working volume(800–2400μL)
•No edge effect
•Continuous vibration operation (without manual intervention)
•Established mass transfer conditions
•Can be scaled up to the scale of a desktop fermentation tank
•data analysis software
•be beneficial forPATandQBD
•The system can be upgraded toBioLector Promodel(Equipped with microfluidic microplates)


Measurement schematic









technical parameter