High pressure freezer Leica EM ICE

Why use frozen fixation?

Currently, freezing fixation is the only way to solidify cellular components without causing significant structural changes.

Why use light stimulation?

Synchronous light stimulation and high-pressure freezing allow you to view structural changes in highly dynamic processes or photosensitive samples with nanometer level resolution and millisecond level accuracy. This will bring new possibilities for researchers in the fields of life sciences and industry.
Apply light stimulation to any photosensitive compound, such as detergents, cosmetics, or food, as well as photoactivated samples (such as proteins or various biological samples)
Drive our understanding of high dynamic processes such as synaptic neurotransmission

Why use Leica EM ICE?

• Because it is the only tool capable of freezing dynamic phenomena on a millisecond scale
• Because it is the only high-pressure freezer that fully integrates light stimulation
• Because its function brings new possibilities for researchers in the fields of life sciences and industry
• Because it is the only high-pressure freezer that allows freezing of 9 subsequent samples
• Because it is the only high-pressure freezer that can provide you with the most comfortable operation

Choose Leica EM ICE; Because it is a platform for you to find disruptive discoveries in your field. New tools, new perspectives.

Five colors

Blue light, ultraviolet light, green light, red light, and amber light: You can use 5 different wavelength LED modules to excite your sample.
The complete integration of light stimulation creates precise relationships between light pulses (i.e. from stimulation to freezing time)
Connect the LED module to the instrument with just one click. Each module has a different wavelength and is automatically recognized by software and recorded in log files
Available optical modules and wavelengths
Blue light: 450Lambda
UV light: 380 Lambda
Green light: 523 Lambda
Red light: 660 Lambda
Amber light: 597 Lambda

Focus on your samples

You only need one step to load samples in Leica EM ICE. When you close the lid of the loading platform, it triggers the perfectly aligned cartridge assembly and freezing process
No additional operation required: The sample addition and freezing process is fully automated, and you can observe and process the sample one second before actual freezing

increase efficiency

With Leica, you can work quickly and efficiently.
Recovering from the refrigeration cycle only takes one minute - no need to wait
Sample storage Dewar automatically refills with LN without maintenance
Use 3 positions that can accommodate up to 3 different samples
Perform up to 9 consecutive freezing cycles
Select the number of samples for each position, programmable rotation and differentiation of samples and conditions
LN Dewar flask automatically drains without operation

Flexible and upgradable at any time

Leica EM ICE brings you the research flexibility you need.
Define your microscope configuration for Leica S6 E or Leica M80 and environmental controls (loading station, workbench, and indoor temperature control)
Upgrade the instrument to light stimulation mode at any time
You can upgrade the environment settings (workbench heating and/or environmental chamber) at any time as needed
All upgrades (light stimulation module and environment settings) can be directly completed by Leica's service team in your laboratory. You don't need to return the instrument to the factory.

Environmental responsibility

Our electron microscope sample preparation system not only meets the highest technical and ergonomic requirements, but is also designed with minimal environmental impact.
The daily LN consumption (including equipment cooling) is only 30 liters, which is approximately 65% less than previous models
The consumption of LN during the cooling process has been reduced by approximately 70% compared to previous models
Synchronization of pressure and cooling does not require the use of alcohol or other liquids
Leica EM ICE adopts sustainable packaging: multiple use packaging boxes containing ramps used during transportation in the factory and delivery to customers. This packaging was awarded the Austrian National Award for "Smart Packaging" in November 2014.
We take responsible actions and obtained DIN EN ISO 14001 environmental management certification in 2015.