High connotation screening system

NegotiableUpdate on 01/27

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

In the drug development market, with the increasing demand for cell-based detection and phenotype screening, there is also a growing need for high content analysis systems for drug efficacy evaluation. In order to improve screening efficiency, higher speed (higher content) equipment is needed. On the other hand, in order to cross the "valley of death" in the drug development process, it is necessary to improve the quality of screening hits. This requires the use of multiple parameters to construct a more complex evaluation system, such as through 3D culture systems, live cell imaging, and combined with more detailed image analysis methods. Balancing the complexity of screening flux and screening evaluation system is an important issue in current development .....

Product Details

CellVoyager CV8000 is aHigh connotation screening systemIt can solve the contradiction point of this screening problem. By combining Yokogawa Electric's proprietary high-speed confocal scanner, immersion objective lens, up to four high field cameras, a stage for maintaining cell culture environment, and an automatic pipette, not only can high-resolution imaging be achieved, but phenotype screening can also be performed through more complex evaluation systems. In addition, Yokogawa Electric's professional analysis software CellPathfinder uses deep learning and machine learning to accurately recognize target objects, which can help you better analyze images and display experimental results with charts.

Exploring the Unknown World

-Real time confocal and label free imaging-

Long term live cell imaging

kinetic analysis

Standard live cell loading platform. Continuous and long-term observation (more than 3 days) is achieved through humidity, temperature, and CO2 control.

By using an automatic pipette with a disposable pipette tip, drugs can be added during the imaging process, making it very suitable for observing rapidly reacting kinetic experiments.

Organoids/Spheres

Unmarked analysis

Even for 3D cultured samples that are difficult to image clearly and quickly, Yokogawa Electric's dual disc confocal technology can effectively capture images of deep samples, achieving evaluations close to in vivo quality.

Recognition and analysis can be performed by obtaining bright field images from multiple Z-axis positions and creating CE bright field images using the accompanying CellPathfinder analysis software. Further improve analysis accuracy through new deep learning features.

High connotation screening system

Dual turntable confocal system

Using Yokogawa Electric's proprietary multi-point scanning method, 1000 laser beams are used to scan the target field of view at high speed through a series of rotating double disks. These turntables consist of a pinhole array turntable (with approximately 20000 pinholes arranged in a helical pattern of equal pitch) and a microlens array turntable that focuses the excitation laser into a single pinhole. This not only enables high-speed imaging, but also largely prevents phototoxicity and fluorescence bleaching.

Capture finer structures

Original water immersion objective lens

Immersion lenses are adept at capturing high-resolution images of cells in liquids. CV8000 can be equipped with 40x or 60x immersion lenses. The Yokogawa 40x lens is a special lens that can perform highly spherical aberration correction, allowing for the capture of bright high-resolution images across the entire wide-angle range. The water supply for the lenses has also been automated. This device can achieve high content screening through a water immersed lens.

Capture the movement of living cells

High precision incubator and automatic pipette

The live cell stage adopts a closed structure, which can control humidity, temperature, and CO2 levels. The automatic pipette can fully automate the following pipetting operations: insert the pipette tip → aspirate reagents from the reagent plate → add reagents to the sample plate → remove the pipette tip. This not only enables rapid acquisition of images before and after reagent infusion, but also allows for multiple additions of reagents and adjustment of addition rates in a single well, thereby expanding the detection mode of kinetic research.

Deeper and clearer observation

Pinhole rotary disc exchanger

Two different types of pinhole turntables (25/50 μ m) can be used based on the sample. For thick samples, reducing the pinhole diameter can obtain finer and clearer images. For darker samples, increasing the pinhole diameter can make the image brighter.

Higher throughput screening

Optical configuration

The optical system configuration can be selected according to the purpose. A 96 well plate can complete imaging of four colors in one minute by connecting four high-sensitivity wide field SCMOS cameras. The system also supports FRET and CellPainting detection.