Hepatitis C virus (HCV) fluorescent PCR detection kit

Product Name: Hepatitis C Virus (HCV) Fluorescent PCR Detection Kit

Name ： Hepatitis C virus Detection Kit(Real-Time PCR Method)

【 Packaging Specification 】 50T/box

Hepatitis C is an infectious disease mainly caused by liver damage due to infection with hepatitis C virus (HCV), and belongs to Class B infectious disease. This kit is suitable for detecting the RNA of hepatitis C virus in patient serum and plasma samples, and is suitable for auxiliary diagnosis of hepatitis C virus infection.

【 Principle of Inspection 】

This kit is designed with specific primers and probes for the conserved region of the hepatitis C virus genome. One step fluorescent RT-PCR technology is used for in vitro amplification and detection of hepatitis C virus RNA, which is used for pathogen detection of suspected infectious materials in scientific research experiments.

【 Reagent Composition 】

Explanation: Components of different batch numbers of reagent kits cannot be used interchangeably.

Storage conditions and expiration date

-20 ℃± 5 ℃, stored in the dark, transported, and subjected to repeated freeze-thaw cycles no more than 5 times, with a validity period of 12 months.

ABI7500、 Agilent MX3000P/3005P, LightCycler, Bio Rad, Eppendorf and other series of fluorescence quantitative PCR detectors.

Serum: Use a disposable syringe to extract 2 mL of venous blood from the subject, place it in a sterilized disposable non anticoagulant test tube with a unique identification number, and directly use the separated serum for testing, storage, or submission. Plasma: Use a disposable syringe to extract 2 mL of venous blood from the subject, place it in a sterilized disposable anticoagulant (non heparin) test tube with a unique identification number, and directly use the separated plasma for testing, storage, or submission. Hemolytic samples should be re sampled. If the triglyceride content exceeds 6 mmol/L or if the plasma or serum appears white and turbid to the naked eye, the sample should be centrifuged at 4 ℃ 16200g for 15 minutes and the clear serum or plasma from the lower layer should be aspirated.

[Storage and Transportation]

The collected or processed samples should be stored at 2 ℃~8 ℃ for no more than 24 hours; if long-term storage is required, they should be stored at -70 ℃ or below, with no more than 3 freeze-thaw cycles.

【 Precautions 】

1.All operations must be strictly carried out in accordance with the instructions;

2.Before use, all components in the reagent kit should be naturally melted, thoroughly mixed, and briefly centrifuged;

3.The reaction solution should be stored away from light;

4.Try to avoid the presence of bubbles during the reaction, and cover the tube tightly;

5.Use disposable suction tips, disposable gloves, and specialized work clothes for each area;

6.Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross contamination;

7.After the experiment is completed, treat the workbench and pipette with 10% hypochlorous acid, 75% alcohol, or a UV lamp;

8.All items in the reagent kit should be treated as contaminants and handled in accordance with the "Biosafety Guidelines for Microbial Biomedical Laboratories".

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